BD™ AbSeq Oligo Mouse Anti-Human FcRL6

The 2H3 monoclonal antibody specifically binds to human Fc receptor-like protein 6 (FCRL6), which is also known as Fc receptor homolog 6 (FcRH6), IFGP family protein 6 (IFGP6) or Immune receptor translocation-associated protein 6 (IRTA6). FCRL6 is a type I transmembrane glycoprotein of the Immunoglobulin (Ig) gene superfamily with 3 extracellular Ig domains. In healthy donors, FCRL6 is expressed by cytolytic CD8-positive T cells and NK cells; its expression is expanded to populations of CD4-positive T cells in HIV-1-positive patients. FCRL6 expression is associated with NK cell maturation. Human FCRL6 binds to HLA-DR, and it has a cytoplasmic immunoreceptor tyrosine-based inhibition motif (ITIM).

Application Notes

The antibody was conjugated to an oligonucleotide that contains an antibody clone-specific barcode (ABC) flanked by a poly-A tail on the 3' end and a PCR handle (PCR primer binding site) on the 5' end.  The ABC for this antibody was designed to be used with other BD AbSeq oligonucleotides conjugated to other antibodies. All AbSeq ABC sequences were selected in silico to be unique from human and mouse genomes, have low predicted secondary structure, and have high Hamming distance within the BD AbSeq portfolio, to allow for sequencing error correction and unique mapping. The poly-A tail of the oligonucleotide allows the ABC to be captured by the BD Rhapsody™ system. The 5' PCR handle allows for efficient sequencing library generation for Illumina sequencing platforms.

NOTE:  The BD Rhapsody Single-Cell Analysis System must be used with the BD Rhapsody Express Instrument.