BD® OMICS-One Comprehensive Immuno-Oncology Protein Panel

This reagent is provided lyophilized in a pre-titrated format. Each test can stain up to 2 million cells.

1.        Remove one tube of BD® OMICS-One T-Cell Protein Panel, one tube of BD® OMICS-One B-Cell Protein Panel, one tube of                              BD® OMICS-One Tumor Protein Panel, one tube of BD® OMICS-One NK-Cell Protein Panel and one tube of BD® OMICS-One         APC/Myeloid-Cell Protein Panel from foil bags and bring up to room temperature for 5 minutes.

2.        Make sure all pellets are located at the bottom of the tubes. If not, briefly centrifuge to collect the contents at the tube bottom.

3.        For each tube, add 35 µL of nuclease-free water to the bottom of the tube and allow antibodies to reconstitute for 5 minutes at room         temperature.

4.        Place the reconstituted antibodies on ice until the cells are ready for staining.

        Note: Reconstitute antibody immediately before cell staining. Prolonged incubation of reconstituted antibodies may increase the                        non-specific background.

        Note: For options for BD® AbSeq Antibody-Oligo drop-in, reach out to technical support for calculation.

5.        Prepare the staining solution with cells using  the table below for substeps a–e.

        _______________________________________________________________________________________________________

        Component                      Co-labeling with Sample Tags       Sequential labeling with Sample Tags

                                                                          or no Sample Tags

                                       Fc Block (µL)  No Fc Block (µL)    Fc Block (µL)  No Fc Block (µL)______

        5‑panel pool (35 µL × 5)       175            175                 175            175

        SMK                            20             20                  0              0

        Cell suspension in Fc Block    25             0                   25             0

        Cell suspension in FBS         0              25                  0              25

        Total staining volume          220            220                 200            200___________________

        Prepare the 5-panel pool by combining the five tubes of 35‑µL rehydrated solutions from step 4.

        a.        For co-labeling with Sample Tags, for each sample, add 20 μL Sample Tag to the 5-panel pool.

        b.        Pipet-mix the mixture, briefly centrifuge to collect the contents at the tube bottom, and place back on ice.

        c.        Centrifuge cells at 400 × g for 5 minutes. If Fc Block is used, proceed to substep d. If Fc Block is not used, skip to substep e.

        d.        (Optional) For samples containing myeloid and B lymphocytes, BD Biosciences recommends blocking nonspecific Fc                                 Receptor–mediated false-positive signals with Human BD Fc Block (catalog number 564220).

                i.        To perform blocking, pipet the Fc Block MasterMix into a new 1.5-mL LoBind tube on ice:

                        _________________________________________________________________________________

                        Component                           1 sample (µL)*    1 sample + 20% overage (µL)

                        FBS† (catalog number 554656)        20.0              24.0

                        Fc Block‡ (catalog number 564220)   5.0               6.0

                        Total                               25.0              30.0                      

                

                        *  Sufficient for up to 1 million cells. To block more cells, adjust the volume.

                        †  FBS = BD Pharmingen™ Stain Buffer.

                        ‡ Fc Block =  BD Pharmingen™ Human BD Fc Block.

                        

                ii.        Pipet-mix the Fc Block MasterMix and briefly centrifuge. Place on ice.

                iii.        Remove the supernatant from the cells without disturbing the pellet.

                iv.        Resuspend the cells in 25 μL of Fc Block MasterMix.

                v.        Incubate the cells at room temperature (15°C to 25°C) for 10 minutes.

                vi.        Add the mixture from substep c to the cell suspension. Pipet-mix and proceed to step 6.

        e.        Remove the supernatant from the cells without disturbing the pellet. Add 25 μL Stain Buffer (FBS) to resuspend the cell pellet.                                 Pipet-mix.

6.        Transfer the cells with BD® AbSeq labeling MasterMix into a new 5-mL polystyrene Falcon tube.

7.        Stain the cells on ice for 30 minutes.

8.        Add 3–4 mL Stain Buffer (FBS) to labelled cells and pipet-mix.

9.        Centrifuge at 400 × g for 5 minutes.

10.        Uncap the tube and invert to decant supernatant into biohazardous waste. Keep the tube inverted and gently blot on a lint-free wiper to          remove residual supernatant from tube rim.

11.        Repeat steps 8–10 twice more for a total of three washes.

12.        Resuspend the final washed cell pellet in 620 µL cold Sample Buffer from the BD Rhapsody™ Enhanced Cartridge Reagent V3
        (catalog number 667052) and proceed to single cell capture with on-cartridge washing described in substeps a–c. Refer to the
        BD Rhapsody™ HT Single-Cell Analysis System Single-Cell Capture and cDNA Synthesis Protocol (Doc ID 23-24252) or BD Rhapsody™

        HT Xpress System Single-Cell Capture and cDNA Synthesis Protocol (Doc ID 23-24253) for additional details.

        Note: Perform on-cartridge washing after cell settling (8-minute incubation) as described in the following substeps.

        a.        At the protocol section of "Loading cells in BD Rhapsody™ 8-Lane Cartridge", after cell load, incubate the cartridge in the dark at                 room temperature for 8 minutes.

        b.        Place the cartridge on the BD Rhapsody™ HT Xpress and perform the On-Cartridge Wash steps as follows:

                _____________________________________________________________

                Material to load        Volume (µL) 1 lane      Pipette Mode

                Air                     380                     Prime/Wash

                Cold Sample Buffer      380                     Prime/Wash

                Air                     380                     Prime/Wash

                Cold Sample Buffer      380                     Prime/Wash  

        c.        (Optional) Perform the scanner step: Cell Load Scan, if using BD Rhapsody™ HT Single-Cell Analysis System Single-Cell Capture

                and cDNA Synthesis Protocol (Doc ID 23-24252). No need for 8-minute delay before scanning.

Warning: All biological specimens and materials are considered biohazardous. Handle as if capable of transmitting infection and dispose using proper precautions in accordance with federal, state, and local regulations. Never pipette by mouth. Wear suitable protective clothing, eyewear, and gloves.

List of all 30 Human AbSeq specificities included in the BD® OMICS-One T-Cell Protein Panel:

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Specificity     Clone      Oligo ID     BD® AbSeq Barcode Sequence___________

CD103**         Ber-ACT8   AHS0001      AAATAGTATCGAGCGTAGTTAAGTTGCGTAGCCGTT

CD137           4B4-1      AHS0003      TGACAAGCAACGAGCGATACGAAAGGCGAAATTAGT

CD45RA          HI100      AHS0009      AAGCGATTGCGAAGGGTTAGTCAGTACGTTATGTTG

CD69            FN50       AHS0010      CAATAACGGGTCATAGTAAGTCGCGAGTAAGAGGGC

CD278           DX29       AHS0012      ATAGTCCGCCGTAATCGTTGTGTCGCTGAAAGGGTT

CD134 (OX40)    ACT35      AHS0013      GGTGTTGGTAAGACGGACGGAGTAGATATTCGAGGT

CD279 (PD-1)†   EH12.1     AHS0014      ATGGTAGTATCACGACGTAGTAGGGTAATTGGCAGT

CD366 (TIM-3)   7D3        AHS0016      TAGGTAGTAGTCCCGTATATCCGATCCGTGTTGTTT

CD223 (Lag3)    T47-530    AHS0018      CGGCATGAATTAGGCGAGACTTAGTATACGAGCTGG

CD95 (Fas)*     DX2        AHS0023      GGCCCGTTAGAGTTGGTATCCGTATGAAGGTTAGCT

CD25            2A3        AHS0026      AGTTGTATGGGTTAGCCGAGAGTAGTGCGTATGATT

CD127           HIL-7R-M21 AHS0028      AGTTATTAGGCTCGTAGGTATGTTTAGGTTATCGCG

CD183           1C6/CXCR3  AHS0031      AAAGTGTTGGCGTTATGTGTTCGTTAGCGGTGTGGG

CD4             SK3        AHS0032      TCGGTGTTATGAGTAGGTCGTCGTGCGGTTTGATGT

CD196 (CCR6)    11A9       AHS0034      ACGTGTTATGGTGTTGTTCGAATTGTGGTAGTCAGT

CD45RO          UCHL1      AHS0036      TGAGAGGTTATTGGGCGTATGACTTCGGTGATTGTG

CD194 (CCR4)    1G1        AHS0038      AATATTAGTGGGTCCTCGCGTTGGCCGGTTGTTAGT

CD62L           DREG-56    AHS0049      ATGGTAAATATGGGCGAATGCGGGTTGTGCTAAAGT

CD272           J168-540   AHS0052      GTAGGTTGATAGTCGGCGATAGTGCGGTTGAAAGCT

CD154           TRAP1      AHS0077      TAAGAGGTAAGTGCATTCGGGTATAGGCGTGATTTG

CD357 (GITR)    V27-580    AHS0104      TCTGTGTGTCGGGTTGAATCGTAGTGAGTTAGCGTG

CD28            L293       AHS0138      TTGTTGAGGATACGATGAAGCGGTTTAAGGGTGTGG

TCRgd           11F2       AHS0142      CTCGTGGGTTAGGCTTGATCGTAGTTATGTATGGTT

CD44†           L178       AHS0167      GTGATTGATTAGGACAGTTCGTTGCTTAGTAGTGGG

TCR Va24-Ja18   6B11       AHS0175      TTCTGGTTCGGTTGAGCTACTAATTTCGTTGGATGG

CD161 (KLRB1)‡  HP-3G10    AHS0205      TTTAGGACGATTAGTTGTGCGGCATAGGAGGTGTTC

CD8             SK1        AHS0228      AGGACATAGAGTAGGACGAGGTAGGCTTAAATTGCT

CD3‡            UCHT1      AHS0231      AGCTAGGTGTTATCGGCAAGTTGTACGGTGAAGTCG

CD197 (CCR7)    2-L1-A     AHS0273      AATGTGTGATCGGCAAAGGGTTCTCGGGTTAATATG

TIGIT           tgMab-2    AHS0411      AGAGGGTTTAGTCAAGGTCGTGCGTATAGTTCAGGT

List of all 30 Human AbSeq specificities included in the BD® OMICS-One B-Cell Panel:

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Specificity     Clone       Oligo ID    BD® AbSeq Barcode Sequence___________          

CD20            2H7         AHS0008     TTGCTTGTTGCGCGTTAGAGAGTATGTCGGGAGATG

CD275           2D3/B7-H2   AHS0011     GTTTATATGTACGACGCCCGGTTGACGAGTGGAAGT

CD38‡‡          HIT2        AHS0022     GTCAACGATGGGTAGCGGTAGAAATAACGGAACTGG

CD95(Fas)*      DX2         AHS0023     GGCCCGTTAGAGTTGGTATCCGTATGAAGGTTAGCT

CD27‡‡          M-T271      AHS0025     TGTCCGGTTTAGCGAATTGGGTTGAGTCACGTAGGT

CD19            SJ25C1      AHS0030     TAGTAATGTGTTCGTAGCCGGTAATAATCTTCGTGG

HLA-DR***       G46-6       AHS0035     TGTTGGTTATTCGTTAGTGCATCCGTTTGGGCGTGG

CD185 (CXCR5)   RF8B2       AHS0039     AGGAAGGTCGATTGTATAACGCGGCATTGTAACGGC

CD24††          ML5         AHS0042     ACTTTGGGTTGAGCGCATGATTATTCGTGACACTTT

CD80***         L307.4      AHS0046     GAGGGTAACGGGTGTCCAAATATCGGCTGTGTAAGT

CD5             UCHT2       AHS0047     ACGAAGCGAGCGAAGAACCTATGCGATTGAGTAAGT

CD10            HI10a       AHS0051     CCTGTTTGATGCGTACGGAGATTTAGCGGATTTATG

IgD             IA6-2       AHS0058     TGAGGGATGTATAGCGAGAATTGCGACCGTAGACTT

IgG             G18-145     AHS0059     AGGTAGGTTATCGTAGGGTAGACTTAGCGGGCATTG

CD184 (CXCR4)‡‡ 12G5        AHS0060     CAGTGTTTAGAGCGGGTTGCATATGTCGTTTAGAGG

CD34††          581         AHS0061     TGGGTGTATTACGGTTAGTTTATGCGCGAAGGTGTT

CD21            B-ly4       AHS0074     GTATTCGCGTATTGTCAGTCGGTAGGGTTATGGTCT

CD9             M-L13       AHS0082     GGGTTGTAAGTCGTCGGAAGTGTGAAGCGTATAGTG

CD126           M5          AHS0096     AATGGTGAATCGCCCTAGCAAGTGGTATCGGAATCG

CD30            BERH8       AHS0114     CCAGTGTAGATTGAGCCGTCGATTTAGTTAGCAGTG

CD40***         5C3         AHS0117     GGTGTAATTGGGCTAGAACGTATATGCGGTAAGGCG

CD138           MI15        AHS0121     TAAGCTGCCGGTATTGGAAACGTATCGATCTATTGG

CD79B           CB3-1       AHS0153     CATCATGAGTAGTTGCTTCGGCGAGTAGGTTTAATT

CD22            HIB22       AHS0195     TGGTTCGTGACTGTATAGGCTTAGCTTAGGCAATTT

IgM             G20-127     AHS0198     TTTGGAGGGTAGCTAGTTGCAGTTCGTGGTCGTTTC

CD43            1G10        AHS0200     ATGGCGGATGGATTTGTCGGTGATATTGCTCTCGTT

CD268 (BAFF-R)  11C1        AHS0206     TGTGAATGAGTTAAGCGTCGCGGATATGTAGAGCCT

CD23            EBVCS-5     AHS0210     TTTGATGTGGGCGGGTTGTATTACGGTTTCGAGTCT

CD73            AD2         AHS0216     AAAGTAGGGTCGATCAAGGGAGTTAACGGTAGCGCT

CD1d            CD1d42      AHS0219     GTTAGGATTATTGACGTACCGAGTTAGGAGTGATTG

List of all 30 Human AbSeq specificities included in the BD® OMICS-One Tumor Protein Panel:

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Specificity     Clone       Oligo ID     BD® AbSeq Barcode Sequence___________

CD274 (PD-L1)‡‡‡ MIH1       AHS0004      ATCGTAAGGCTCGTGGTTCGTAAGTAAGTTCGTATC

CD279 (PD-1)†   EH12.1      AHS0014      ATGGTAGTATCACGACGTAGTAGGGTAATTGGCAGT

CD45‡‡‡         HI30        AHS0040      GTGCGAAATGGCGGAATGTTATCTGCGAATGTAGTC

CD324 (E-cad)   67A4        AHS0041      GATATGAATGGGTTGCGGTGTAAAGTCGTAATGGTT

CD24††          ML5         AHS0042      ACTTTGGGTTGAGCGCATGATTATTCGTGACACTTT

CD90            5E10        AHS0045      GACTATATGTACGGTGTTAATTCGGGATCCTGCGCT

CD34††          581         AHS0061      TGGGTGTATTACGGTTAGTTTATGCGCGAAGGTGTT

CD117           YB5.B8      AHS0064      GGATTAGTTGTCGTTATAGGGAGTGCGTTCTTAGCG

HLA-A,B,C       G46-2.6     AHS0066      GATATGCATGGCGAGTAGGTAGAACGAAGCTTAGGT

CD54†††         HA58        AHS0076      AAGAGAATATATGCGTGCGTTGTTAAGGGAATGCGT

CD29            MAR4        AHS0080      TGGTAAGGTGGTTGCGAGTAAGTAGCGGTGAGTTGT

CD47            B6H12       AHS0087      TGTTAGGTTCGACGTATTATGTGTAGATCCGCAAGG

CD326 (EpCam)   EBA-1       AHS0089      TTGAGCGTAAAGTTGCGTCCGGTAATTGAGTTGCGT

CD66            B1.1/CD66   AHS0094      GTCTGCGCAAGGTAAGCTAAGTAACGAAAGGGATCT

CD133           W6B3C1      AHS0103      TTTGGTATTGGCACGGTTTGTAGCGAGTTGACGGTC

CD26            M-A261      AHS0109      TGTAGGTTGCGCGGTTATTAGGGTATTATCGATCTG

CD155           TX24        AHS0111      GCGGTGGATCGATGGGTATAGTTGGTAATTTGCGTC

CD146           P1H12       AHS0127      AGGTTATTTAGGTGACGGTTGTATTGACGAGAGAGG

C-MET           3D6         AHS0132      AGCGTGAGTTGTCGGTAGTTAATTATCGGAGAGTTT

ITGRN BTA 7     FIB504      AHS0158      TTTCAGTTTGGTCGCAGTTAAGGTATCGTATGGGTC

CD44            L178        AHS0167      GTGATTGATTAGGACAGTTCGTTGCTTAGTAGTGGG

CD31 (PECAM1)††† WM59       AHS0170      CTAAGGGACGTAATTGAGTTTCGGTGATCGCAGTTT

EphB2           2H9         AHS0176      TATTGCGGGTAGGATTTGTCTCGAAGCGTAGGTAGC

Vista‡‡‡        MIH65.RMAB  AHS0187      ATCAGGGAATCTCGGTAAGTTAAACGTGTATAGTGC

PDPLN           LPMAB-17    AHS0192      TTTATGAGTATTACGTCTGTTGCGATTGTTGGCGGT

NOTCH1          MHN1-519    AHS0214      CGTAGTAGGAGCGTGTTTCATCGGCATTATCGTTTG

CD325 (n-Cad)   8C11        AHS0223      TAGGATGAGTTTCGTAAGTAAGGTAGTCGTATGGCT

CD58            1C3         AHS0237      TTGGTGAGTATTGGTGCGTAGTATGCGGGATGTTTG

EGFR            EGFR.1      AHS0241      ATATGATTGATGCGGGTTAGCCTACAGATTCGAGTT

CD227 (MUC1)    HMFG2       AHS0247      AGTGCATGGTTAGTAGGTGTGAGTCGTTAGATATTC

List of all 30 Human AbSeq specificities included in the BD® OMICS-One NK-Cell panel:

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Specificity     Clone       Oligo ID     BD® AbSeq Barcode Sequence___________          

CD11b****       M1/70       AHS0005      ATCGTTATTCGTTGTAGTTCGCCCGGTTTGAGTAGT

CD56            NCAM16.2    AHS0019      AGAGGTTGAGTCGTAATAATAATCGGAAGGCGTTGG

CD38‡‡          HIT2        AHS0022      GTCAACGATGGGTAGCGGTAGAAATAACGGAACTGG

CD27‡‡          M-271       AHS0025      TGTCCGGTTTAGCGAATTGGGTTGAGTCACGTAGGT

CD2             RPA-2.10    AHS0029      AAACGTAGATTAGAGCCGGGTATGTCGCAACTGATT

CD16****        3G8         AHS0053      TAAATCTAATCGCGGTAACATAACGGTGGGTAAGGT

CD184 (CXCR4)‡‡ 12G5        AHS0060      CAGTGTTTAGAGCGGGTTGCATATGTCGTTTAGAGG

CD49d           9F10        AHS0063      TAGGGTGACTTAGCGATTGATGCGTATGTTTGGGCG

CD314 (NKG2D)   1D11        AHS0065      TTGAAATGCGATGAGACGTAGAGCGATGTAGGTAGC

CD335 (NKP46)   9E2/NKP46   AHS0068      CAATTTGTTCGCGTTTAGTAGTCGTCGTCTTATGGG

CD54†††         HA58        AHS0076      AAGAGAATATATGCGTGCGTTGTTAAGGGAATGCGT

CD226           DX11        AHS0079      GAGTTTATGATTCGTTTCTTCGGTAGTTCGTCGCTT

CD94            HP-3D9      AHS0085      GAGGTTAGGATAGGTGTACGGGTCGAGTTGAATTCT

CD336 (NKP44)   p44-8       AHS0090      AATGCAAACGATATCACGAAGGGTAGTACACGACGG

CD49a           SR84        AHS0101      ATGACACGAATGCGACGAGAGGCGAAATAGGTTGGT

CX3CR1          2A9-1       AHS0125      GGGTTCACGAGGTTTAAAGCGGTAGTATAGGATGCC

CD122           Mik-β3      AHS0146      TTAAAGAGATTCGTGGGTATTGGCGCAGTCATTCCT

CD140b (PDGFR)  28D4        AHS0151      GACAACATTTAGGACGTGACGAGAGAGTATAGCTTC

CD248           B1/35       AHS0156      ATCACTTATTTCGTTTGGAGGGTTCGTAGGCGTTGC

CD63            H5C6        AHS0157      TGCAGCGTTAGGACCAAGCGTTTACCGTAGAATATT

CD140a          α-R1        AHS0160      TTACTGACTTTCGGACGTTGGTTACTTAGGGTTATG

CD31 (PECAM1)††† WM59       AHS0170      CTAAGGGACGTAATTGAGTTTCGGTGATCGCAGTTT

CD96            6F9         AHS0194      CTAATGTAAGAGCGGACGTTTGGGCACTATATGTTT

CD161 (KLRB1)‡  HP-3G10     AHS0205      TTTAGGACGATTAGTTGTGCGGCATAGGAGGTGTTC

CD158b (KIR)    DX27        AHS0209      CGTAGGAGGATTTCGTCGATGGGTTTGTTAGCGTTC

CD158e1         DX9         AHS0211      AGGTTCATTGCGGCATTAGGCGTCATATAGTAGGTG

CD337/NKp30     P30-15      AHS0213      GGTAACTGACATGACGGAGCGATAATTTCTGGCGGT

CD3‡            UCHT1       AHS0231      AGCTAGGTGTTATCGGCAAGTTGTACGGTGAAGTCG

CD329 (Siglec-9) E10-286    AHS0239      CGGGCGCGAAGATAGGATAATAGGTAACGTCAAATG

CD106           51-10C9     AHS0251      TCTGATTTAGCGGGTGGACGTATTATAGTGATTGGC_

List of all 30 Human AbSeq specificities included in the BD® OMICS-One APC/Myeloid-Cell panel:

______________________________________________________________________________

Specificity     Clone       Oligo ID     BD® AbSeq Barcode Sequence___________          

CD103**         BER-ACT8    AHS0001      AAATAGTATCGAGCGTAGTTAAGTTGCGTAGCCGTT

CD274 (PD-L1)‡‡‡ MIH1       AHS0004      ATCGTAAGGCTCGTGGTTCGTAAGTAAGTTCGTATC

CD11b****       M1/70       AHS0005      ATCGTTATTCGTTGTAGTTCGCCCGGTTTGAGTAGT

CD123           7G3         AHS0020      ACAGTTTAGTAGGACGTGAGGTATCGCGAGAATGCC

HLA-DR***       G46-6       AHS0035      TGTTGGTTATTCGTTAGTGCATCCGTTTGGGCGTGG

CD14            MPHIP9      AHS0037      TGGCCCGTGGTAGCGCAATGTGAGATCGTAATAAGT

CD45‡‡‡         HI30        AHS0040      GTGCGAAATGGCGGAATGTTATCTGCGAATGTAGTC

CD33            WM53        AHS0044      GTGTTAGTGATTTGATAGGACGCGTTACGAGAGATT

CD80***         L307.4      AHS0046      GAGGGTAACGGGTGTCCAAATATCGGCTGTGTAAGT

CD16****        3G8         AHS0053      TAAATCTAATCGCGGTAACATAACGGTGGGTAAGGT

CD64            10.1        AHS0055      TTGTGCGGCGTAGTATGGTTATCTCGAGTGAAAGTC

CD11c           B-LY6       AHS0056      ATGCGTTGCGAGAGATATGCGTAGGTTGCTGATTGG

CD163           GHI/61      AHS0062      TATTATGTGCGAACTATGGTATCCGTATTGAGGGCT

CD195 (CCR5)    2D7/CCR5    AHS0070      ATGGTTTAGTCGTACGTGGGTTTAGATTGGCGGTGC

CD206           19.2        AHS0072      GCTGGTTATCGTTTGAGAGTCGGTATGGAATGCGGT

CD32            FLI8.26     AHS0073      GGTTGTAGGTGCGGAATATAAGCGTCGTTGAGGTGT

CD273           MIH18       AHS0075      TGAGTAACCGTATGTAATCCGTAATCGTAGAAGCGC

CD141           1A4         AHS0083      TGGAAGTAAGTATGGGTCGGCGTAAATTGTGCGTGT

CD1c            F10/21A3    AHS0088      ATAGATTACATTCGTTTAGCGTTGGGTTCGGTCCGT

CD40***         5C3         AHS0117      GGTGTAATTGGGCTAGAACGTATATGCGGTAAGGCG

FCeR1a          AER-37      AHS0129      GATATGGCGTGATGGTAGGTTCGGTTTAAGTTAGCG

CD169           7-239       AHS0133      CATTAAGCACGAAGGGTATAGGTAGGAACGGTTGGC

CD36            IVC7        AHS0135      AATTGTAGTAGTCCGGTGTATGTAGAGTAGGCGTTT

CD115 (CSF1R)   9-4D2-1E4   AHS0136      CTGGTGGCGGCGAATTTGGTTACGACATATAGGGTT

CD162           KPL-1       AHS0139      CCAGATAGGCGATAGTGTTTAGGAGCGATTAGTGTG

CD85K           ZM3.8       AHS0179      AGTAGTCGTAGTTGGCGTGAATTGGGCTTATATCTG

VISTA‡‡‡        MIH65.RMAB  AHS0187      ATCAGGGAATCTCGGTAAGTTAAACGTGTATAGTGC

CD15            W6D3        AHS0196      ATAGGCATGGACGACGTAGATAATAAGTGGCGGGTT

CD192 (CCR2)    LS132.1D9   AHS0208      CATGAGTGAGGCGATATAGTGAGCGGTTTGTAGATT

CD116           Hgmcsfr1-M1 AHS0238      CTTAGTTGTAGGATCGAGAGTAGGTGTGCATTGCGT_

*        T-Cell and B-Cell Protein Panels contain the same CD95 antibody.

†        T-Cell and Tumor-Cell Protein Panels contain the same CD279 (PD-1) and CD44 antibody.

‡        T-Cell and NK-Cell Protein Panels contain the same CD161 (KLRB1), and CD3 antibody.

**        T-Cell and APC/Myeloid -Cell Protein Panels contain the same CD103 antibody.

††        B-Cell and Tumor-Cell Protein Panels contain the same CD24 and CD34 antibody.

‡‡        B-Cell and NK-Cell Protein Panels contain the same CD27, CD38, and CD184 (CXCR4) antibody.

***        B-Cell and APC/Myeloid -Cell Protein Panels contain the same HLA-DR, CD40, and CD80 antibody.

†††        Tumor and NK-Cell Protein Panels contain the same CD54 and CD31 (PECAM1) antibody.

‡‡‡        Tumor and APC/Myeloid-Cell Protein Panels contain the same CD274 (PD-L1), CD45, and VISTA antibody.

****        NK-Cell and APC/Myeloid-Cell Protein Panels contain the same CD11b and CD16 antibody.

1. This reagent is provided lyophilized in a pre-titrated format.
2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
3. Go to https://www.bdbiosciences.com/en-us/resources/protocols/single-cell-multiomics for technical protocols.
4. Go to https://abseq-ref-gen.genomics.bd.com/to access AbSeq reference files in FASTA format for bioinformatics analyses.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Follow state and local guidelines when disposing of hazardous waste.
6. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
7. For U.S. patents that may apply, see bd.com/patents.
8. Read and understand the safety data sheets (SDSs) before handling chemicals.To obtain SDSs, go to regdocs.bd.com or contact BD Biosciences technical support at scomix@bd.com.