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Purified Mouse Anti-Vimentin
Purified Mouse Anti-Vimentin

Western blot analysis of vimentin. HeLa cell lysate was probed with anti-vimentin (clone RV202, Cat. No. 550513) at concentrations of 120 (lane 1), 60 (lane 2) and 30 ng/ml (lane 3). Vimentin is identified as a band of ~57 kDa.

Western blot analysis of vimentin. HeLa cell lysate was probed with anti-vimentin (clone RV202, Cat. No. 550513) at concentrations of 120 (lane 1), 60 (lane 2) and 30 ng/ml (lane 3). Vimentin is identified as a band of ~57 kDa.

Product Details
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BD Pharmingen™
Human (QC Testing), Rat, Mouse, Hamster, Monkey, Dog, Chicken, Rabbit, Cow, Goat (Reported)
Mouse IgG1
Purified Cow Lens Vimentin
Western blot (Routinely Tested), Electron microscopy, Immunofluorescence, Immunohistochemistry (Reported)
57 kDa
0.5 mg/ml
AB_393716
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
550513 Rev. 1
Antibody Details
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RV202

Intermediate filaments (IF) are a subset of cytoskeletal proteins which function to give overall structural integrity to the plasma membrane as well to organize cells into specific tissues. IF proteins can be divided into six major types based upon the similarity in sequence. Vimentin belongs to the type III category of IF proteins which are expressed in leukocytes, blood vessel endothelial cells, some epithelial cells, and mesenchymal cells. Vimentin is also expressed together with several other IF proteins during the early stages of development. Vimentin is exchanged for the tissue-specific intermediate filament type as differentiation proceeds. Vimentin migrates in SDS/PAGE as a ~57 kDa protein.

550513 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
550513 Rev.1
Citations & References
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Development References (5)

  1. Broers JL, Carney DN, Klein Rot M, et al . Intermediate filament proteins in classic and variant types of small cell lung carcinoma cell lines: a biochemical and immunochemical analysis using a panel of monoclonal and polyclonal antibodies. J Cell Sci. 1986; 83:37-60. (Clone-specific: Immunofluorescence, Western blot). View Reference
  2. Lodish HF. Molecular cell biology, 4th ed.. New York: W.H. Freeman; 2000:795-847.
  3. Pieper FR, Schaart G, Krimpenfort PJ, et al. Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells. J Cell Biol. 1989; 108(3):1009-1024. (Clone-specific: Electron microscopy, Immunofluorescence, Immunohistochemistry, Western blot). View Reference
  4. Raats JM, Pieper FR, Vree Egberts WT, Verrijp KN, Ramaekers FC, Bloemendal H. Assembly of amino-terminally deleted desmin in vimentin-free cells. J Cell Biol. 1990; 111(5):1971-1985. (Clone-specific: Immunohistochemistry). View Reference
  5. Viebahn C, Lane EB, Ramaekers FC. Keratin and vimentin expression in early organogenesis of the rabbit embryo. Cell Tissue Res. 1988; 253(3):553-562. (Clone-specific: Immunofluorescence). View Reference
View All (5) View Less
550513 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.