BD Pharmingen™ Purified Mouse Anti-Human c-Myc with Control
(RUO)
Western blot analysis of c-myc. Lysate from Jurkat cells was probed with anti-c-myc (clone 9E10, Comp. No. 51-1485GR) at concentrations of 2.0 (lane 1), 1.0 (lane 2), and 0.5 µg/ml (lane 3). C-myc is identified as a band of 62 kDa.
Western blot analysis of c-myc. Lysate from Jurkat cells was probed with anti-c-myc (clone 9E10, Comp. No. 51-1485GR) at concentrations of 2.0 (lane 1), 1.0 (lane 2), and 0.5 µg/ml (lane 3). C-myc is identified as a band of 62 kDa.
Product Details
BD Pharmingen™
Human (QC Testing)
Western blot (Routinely Tested), Electron microscopy, Fluorescence microscopy, Immunohistochemistry-paraffin (Reported), Immunohistochemistry-frozen (Not Recommended)
RUO
AB_394046
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Recommended Assay Procedures
It is particularly useful for western blot analysis of myc-tagged recombinant proteins, and recombinant c-myc. Jurkat control lysate [50 µg (1 µg/µl)] is provided as a western blot positive control (Comp. No. 51-16526N; store lysate at -20 °C). Additional control lysate is sold separately.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
551102 Rev. 3
Components
| Description | Quantity/Size | Part Number | EntrezGene ID |
|---|---|---|---|
| Purified Mouse Anti-Human c-Myc | 50 µg (1 ea) | 51-1485GR | N/A |
| Jurkat Cell Lysate | 50 µg (1 ea) | 51-16526N | N/A |
551102 Rev. 3
Citations & References
Development References (13)
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Blackwood EM, Eisenman RN. Max: a helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex with Myc. Science. 1991; 251(4998):1211-1217. (Biology). View Reference
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Campbell AM, Kessler PD, Fambrough DM. The alternative carboxyl termini of avian cardiac and brain sarcoplasmic reticulum/endoplasmic reticulum Ca(2+)-ATPases are on opposite sides of the membrane. J Biol Chem. 1992; 267(13):9321-9325. (Clone-specific: Fluorescence microscopy, Western blot). View Reference
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DePinho RA, Schreiber-Agus N, Alt FW. myc family oncogenes in the development of normal and neoplastic cells. Adv Cancer Res. 1991; 57:1-46. (Biology). View Reference
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Evan GI, Lewis GK, Ramsay G, Bishop JM. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol. 1985; 5(12):3610-3616. (Immunogen: Western blot). View Reference
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Gosney JR, Field JK, Gosney MA, Lye MD, Spandidos DA, Butt SA. c-myc oncoprotein in bronchial carcinoma: expression in all major morphological types. Anticancer Res. 1990; 10(3):623-628. (Clone-specific: Electron microscopy, Immunohistochemistry). View Reference
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Karayiannis M, Yiagnisis M, Papadimitriou K, Field JK, Spandidos DA. Evaluation of the ras and myc oncoproteins in benign gastric lesions. Anticancer Res. 1990; 10(5A):1127-1134. (Clone-specific: Immunohistochemistry). View Reference
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Liu ZG, Hsu H, Goeddel DV, Karin M. Dissection of TNF receptor 1 effector functions: JNK activation is not linked to apoptosis while NF-kappaB activation prevents cell death. Cell. 1996; 87(3):565-576. (Clone-specific: Western blot). View Reference
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Lukas J, Parry D, Aagaard L, et al. Retinoblastoma-protein-dependent cell-cycle inhibition by the tumour suppressor p16. Nature. 1995; 375(6531):503-506. (Clone-specific: Western blot). View Reference
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Royds JA, Sharrard RM, Wagner B, Polacarz SV. Cellular localisation of c-myc product in human colorectal epithelial neoplasia. J Pathol. 1992; 166(3):225-233. (Clone-specific: Immunohistochemistry). View Reference
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Spandidos DA, Yiagnisis M, Papadimitriou K, Field JK. ras, c-myc and c-erbB-2 oncoproteins in human breast cancer. Anticancer Res. 1989; 9(5):1385-1394. (Clone-specific: Immunohistochemistry). View Reference
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Tiniakos D, Spandidos DA, Kakkanas A, Pintzas A, Pollice L, Tiniakos G. Expression of ras and myc oncogenes in human hepatocellular carcinoma and non-neoplastic liver tissues. Anticancer Res. 1989; 9(3):715-722. (Clone-specific: Immunohistochemistry). View Reference
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Tosca A, Linardopoulos S, Malliri A, Hatziolou E, Nicolaidou A, Spandidos DA. Implication of the ras and myc oncoproteins in the pathogenesis of mycosis fungoides. Anticancer Res. 1991; 11(4):1433-1438. (Clone-specific: Immunohistochemistry). View Reference
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Williams AR, Piris J, Wyllie AH. Immunohistochemical demonstration of altered intracellular localization of the C-Myc oncogene product in human colorectal neoplasms. J Pathol. 1990; 160(4):287-293. (Clone-specific: Immunohistochemistry). View Reference
551102 Rev. 3
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
