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Purified Mouse Anti-Rab11
Product Details
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BD Transduction Laboratories™
Dog (QC Testing), Human, Mouse, Rat, Chicken (Tested in Development)
Mouse IgG2a
Human Rab11 aa. 86-207
Western blot (Routinely Tested), Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
24 kDa
250 µg/ml
AB_397984
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
610656 Rev. 1
Antibody Details
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47/Rab11

The Rab proteins are small GTP-binding molecules that are localized to specific intracellular vesicles and organelles. It has been proposed that Rab proteins cycle between GTP- and GDP-bound forms and that this is related to their function as regulators of vesicular traffic. The Rab11 gene encodes a 24 kDa protein of 214 amino acids that has been detected in liver, brain, testis, spleen, and heart. Rab11 protein was isolated from the golgi-microsomal fraction of rat liver and has been detected in the Trans-golgi Network, secretory vesicles, and the pericentriolar recycling endosomes. The distribution of Rab11 indicates that this small protein is involved in regulating traffic at the Golgi complex.

610656 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610656 Rev.1
Citations & References
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Development References (5)

  1. Choudhury A, Dominguez M, Puri V, et al. Rab proteins mediate Golgi transport of caveola-internalized glycosphingolipids and correct lipid trafficking in Niemann-Pick C cells. J Clin Invest. 2002; 109(12):1541-1550. (Clone-specific: Western blot). View Reference
  2. Lai F, Stubbs L, Artzt K. Molecular analysis of mouse Rab11b: a new type of mammalian YPT/Rab protein. Genomics. 1994; 22(3):610-616. (Biology). View Reference
  3. Sakurada K, Uchida K, Yamaguchi K, et al. Molecular cloning and characterization of a ras p21-like GTP-binding protein (24KG) from rat liver. Biochem Biophys Res Commun. 1991; 177(3):1224-1232. (Biology). View Reference
  4. Steiner P, Sarria JC, Glauser L, Magnin S, Catsicas S, Hirling H. Modulation of receptor cycling by neuron-enriched endosomal protein of 21 kD. J Cell Biol. 2002; 157(7):1197-1209. (Clone-specific: Immunofluorescence, Western blot). View Reference
  5. Woods AJ, Roberts MS, Choudhary J, et al. Paxillin associates with poly(A)-binding protein 1 at the dense endoplasmic reticulum and the leading edge of migrating cells. J Biol Chem. 2002; 277(8):6428-6437. (Clone-specific: Western blot). View Reference
View All (5) View Less
610656 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.