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      Purified Mouse Anti-L1 Protein of Human Papilloma Virus
      Purified Mouse Anti-L1 Protein of Human Papilloma Virus
      Immunohistochemical staining for human papilloma virus (HPV) type 16, L1 protein. A formalin-fixed, paraffin-embedded tissue section from human cervix infrected with HPV type 16 was stained with either the Purified Mouse Anti-HPV type 16 L1 antibody (Left panel ) or with a mouse IgG2a isotype control (Right panel).
      Purified Mouse Anti-L1 Protein of Human Papilloma Virus
      Immunohistochemical staining for human papilloma virus (HPV) type 16, L1 protein. A formalin-fixed, paraffin-embedded tissue section from human cervix infrected with HPV type 16 was stained with either the Purified Mouse Anti-HPV type 16 L1 antibody (Left panel ) or with a mouse IgG2a isotype control (Right panel).
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      BD Pharmingen™
      Human (QC Testing)
      Mouse IgG2a, κ
      Immunohistochemistry-formalin (antigen retrieval required) (Routinely Tested), Immunofluorescence, Western blot (Reported)
      0.5 mg/ml
      AB_395284
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      554171 Rev. 9
      Antibody Details
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      CAMVIR-1

      More than 60 different types of human papilloma viruses (HPVs) have been isolated. The human papilloma viruses encode three late proteins, which are produced only in terminally differentiating keratinocytes, two of which (the L1 and L2 proteins) are structural components of the virion.  Virus-like particles can be assembled by over-expressing L1 and L2 in vitro, demonstrating that L1 and L2 are necessary and sufficient components of the HPV capsomeres.  HPV-16 has been reported to migrate at a reduced molecular weight of ~57 kD by SDS-PAGE. The CAMVIR-1 antibody reacts with the L1 protein of HPV-16 and may also cross-react with other HPV types, such as HPV-33.

      554171 Rev. 9
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      554171 Rev.9
      Citations & References
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      View product citations for antibody "554171" on CiteAb

      Development References (4)

      1. Doorbar J, Ely S, Coleman N, Hibma M, Davies DH, Crawford L. Epitope-mapped monoclonal antibodies against the HPV16E1--E4 protein. Virology. 1992; 187(1):353-359. (Biology). View Reference
      2. McLean CS, Churcher MJ, Meinke J. Production and characterisation of a monoclonal antibody to human papillomavirus type 16 using recombinant vaccinia virus. J Clin Pathol. 1990; 43(6):488-492. (Immunogen: Immunofluorescence, Western blot). View Reference
      3. Zhou J, Sun XY, Stenzel DJ, Frazer IH. Expression of vaccinia recombinant HPV 16 L1 and L2 ORF proteins in epithelial cells is sufficient for assembly of HPV virion-like particles. Virology. 1991; 185(1):251-257. (Biology). View Reference
      4. de Villiers EM. Heterogeneity of the human papillomavirus group. J Virol. 1989; 63(11):4898-4903. (Biology). View Reference
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      554171 Rev. 9

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.