BD Transduction Laboratories™ Purified Mouse Anti-Human IGFBP-3
Clone 4/IGFBP-3 (RUO)
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
.png?imwidth=320)
Insulin-like growth factors (IGF) I and II are peptide hormones that regulate cellular proliferation and differentiation. Most circulating IGFs exist in 130-150 kDa ternary complexes containing IGF-Binding Protein-3 (IGFBP-3) and an 85 kDa glycoprotein, the acid-labile subunit (ALS). IGFBP-3 is one of six IGFBPs that, by binding IGF peptides, prolong their half life and maintain the IGF reservoir. IGFBP-3 is found in body fluids as multiple 40-50 kDa forms as a result of differential glycosylation. The expression of IGFBP-3 in many tissues suggests that it locally modulates the autocrine/paracrine action of IGF peptides. IGFBP-3 binding to fibrinogen may be important for wound healing, since this concentrates IGF-I at wound sites and lowers the affinity of IGF-I for IGFBP-3. In addition, retinoic acid-induced IGFBP-3 expression inhibits the growth promoting effects of IGF-I in breast cancer cells. This may link the retinoid and IGF systems in cell growth regulation and explain how the loss of retinoic acid receptor β leads to breast cancer progression. Thus, IGFBP-3 maintenance and regulation of IGF activity in various tissues may have diverse physiological roles.
Development References (4)
-
Campbell PG, Durham SK, Hayes JD, Suwanichkul A, Powell DR. Insulin-like growth factor-binding protein-3 binds fibrinogen and fibrin. J Biol Chem. 1999; 274(42):30215-30221. (Biology). View Reference
-
Cubbage ML, Suwanichkul A, Powell DR. Insulin-like growth factor binding protein-3. Organization of the human chromosomal gene and demonstration of promoter activity. J Biol Chem. 1999; 265(21):12642-12649. (Biology). View Reference
-
Janosi JB, Firth SM, Bond JJ, Baxter RC, Delhanty PJ. N-Linked glycosylation and sialylation of the acid-labile subunit. Role in complex formation with insulin-like growth factor (IGF)-binding protein-3 and the IGFs. J Biol Chem. 1999; 274(9):5292-5298. (Biology). View Reference
-
Shang Y, Baumrucker CR, Green MH. Signal relay by retinoic acid receptors alpha and beta in the retinoic acid-induced expression of insulin-like growth factor-binding protein-3 in breast cancer cells. J Biol Chem. 1999; 274(25):18005-18010. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.