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Purified Mouse Anti-CtBP2
Purified Mouse Anti-CtBP2

Western blot analysis of CtBP2 on a BC3H1 cell lysate (Mouse brain smooth muscle-like cells; ATCC CRL-1443). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-CtBP2 antibody.

Purified Mouse Anti-CtBP2

Immunofluorescence staining of SK-N-MC (Human neuroblastoma; ATCC HTB-10).

Western blot analysis of CtBP2 on a BC3H1 cell lysate (Mouse brain smooth muscle-like cells; ATCC CRL-1443). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-CtBP2 antibody.

Immunofluorescence staining of SK-N-MC (Human neuroblastoma; ATCC HTB-10).

Product Details
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BD Transduction Laboratories™
C-Terminal Binding Protein-2
Mouse (QC Testing), Human, Rat, Dog (Tested in Development)
Mouse IgG1
Mouse CtBP2 aa. 361-445
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
48 kDa
250 µg/ml
AB_399431
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612044 Rev. 1
Antibody Details
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16/CtBP2

Transcriptional repression is critical for genetic regulation of many cellular  proteins, and involves either a transcription regulator that has an intrinsic repressor domain, or a transcription regulator that contains a binding site in the regulatory domain where a co-repressor can bind.  C-terminal binding proteins (CtBP1 and CtBP2) are a class of co-repressors that interact with a subset of transcription factors through a PLDLSL sequence motif.  CtBP1 and CtBP2 are 80% homologous, and both are expressed at high levels in embryonic tissues.  However, CtBP1 is expressed higher in adult tissues, and is more widely expressed in embryonic and adult tissues.  Both CtBP1 and CtBP2 bind the δEF1 zinc finger-homeodomain transcription factor, and enhance transcriptional repression via interaction with the PLDLSL-sequence in δEF1.  CtBP1 and CtBP2 bind other zinc finger transcription factors, including Kheper and BKLF.  In addition, CtBP2 interacts with other transcription factors, such as hFOG-2, Evi-1, AREB6, and ZEB.  Thus, CtBP1 and CtBP2 may be important co-repressors for a variety of transcriptional factors.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

612044 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612044 Rev.1
Citations & References
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Development References (5)

  1. Furusawa T, Moribe H, Kondoh H, Higashi Y. Identification of CtBP1 and CtBP2 as corepressors of zinc finger-homeodomain factor deltaEF1. Mol Cell Biol. 1999; 19(12):8581-8590. (Biology). View Reference
  2. Hildebrand JD, Soriano P. Overlapping and unique roles for C-terminal binding protein 1 (CtBP1) and CtBP2 during mouse development. Mol Cell Biol. 2002; 22(15):5296-5307. (Biology: Immunohistochemistry, Western blot). View Reference
  3. Katsanis N, Fisher EM. A novel C-terminal binding protein (CTBP2) is closely related to CTBP1, an adenovirus E1A-binding protein, and maps to human chromosome 21q21.3. Genomics. 1998; 47(2):294-299. (Biology). View Reference
  4. Muraoka O, Ichikawa H, Shi H. Kheper, a novel ZFH/deltaEF1 family member, regulates the development of the neuroectoderm of zebrafish (Danio rerio). Dev Biol. 2000; 228(1):29-40. (Biology). View Reference
  5. Turner J, Crossley M. Cloning and characterization of mCtBP2, a co-repressor that associates with basic Kruppel-like factor and other mammalian transcriptional regulators. EMBO J. 1998; 17(17):5129-5140. (Biology). View Reference
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612044 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.