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Western blot analysis of AKAP149 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- AKAP149 antibody.
Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).
BD Transduction Laboratories™ Purified Mouse Anti-AKAP149
BD Transduction Laboratories™ Purified Mouse Anti-AKAP149
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Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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The targeting of protein kinases and phosphatases to cellular compartments in a timely manner may provide specificity in signal transduction events. For instance, attachment of type II cAMP-dependent Protein Kinase (PKA) to the cytoskeleton occurs through the binding of the regulatory subunit (RII) to microtubule associated-protein 2. Several proteins, such as microtubule-associated protein 2, have been identified as PKA type I/II anchoring proteins and form a family named AKAP (A-Kinase Anchor Proteins). AKAP149 is a protein of 903 amino acids. It has a PKA [RII] binding site at amino acids 355-376 preceded by a leucine zipper domain. AKAP149 mRNA has been reported to be expressed ubiquitously, with the highest levels in prostate and small intestine. AKAP149 contains a KH domain characteristic of RNA-binding proteins. Thus, AKAP149 is a PKA [RII] anchoring protein that may target this kinase to sites of RNA processing for regulation of RNA binding protein activity.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (5)
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Dell'Acqua ML, Scott JD. Protein kinase A anchoring. J Biol Chem. 1997; 272(20):12881-12884. (Biology). View Reference
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Schillace RV, Andrews SF, Liberty GA, Davey MP, Carr DW. Identification and characterization of myeloid translocation gene 16b as a novel a kinase anchoring protein in T lymphocytes. J Immunol. 2002; 168(4):1590-1599. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
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Steen RL, Collas P. Mistargeting of B-type lamins at the end of mitosis: implications on cell survival and regulation of lamins A/C expression. J Cell Biol. 2001; 153(3):621-626. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
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Tanji C, Yamamoto H, Yorioka N, Kohno N, Kikuchi K, Kikuchi A. A-kinase anchoring protein AKAP220 binds to glycogen synthase kinase-3beta (GSK-3beta ) and mediates protein kinase A-dependent inhibition of GSK-3beta. J Biol Chem. 2002; 277(40):36955-36961. (Biology: Immunoprecipitation, Western blot). View Reference
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Trendelenburg G, Hummel M, Riecken EO, Hanski C. Molecular characterization of AKAP149, a novel A kinase anchor protein with a KH domain. Biochem Biophys Res Commun. 1996; 225(1):313-319. (Biology). View Reference
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