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Human IgG4 Flex Set

BD™ Cytometric Bead Array (CBA) Human IgG4 Flex Set

561118
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$182.00
EA (1 Each)
100 Tests
Product Details
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BD™ Cytometric Bead Array (CBA)
1.6-400 ng/mL
Human (QC Testing)
RUO
AB_2869242


Description

The BD™ CBA Human IgG4 Flex Set is a bead-based immunoassay capable of measuring human immunoglobulin G subclass 4 (IgG4) in serum and plasma samples. Human reactivity was determined by testing samples with the BD CBA Human IgG4 Flex Set. The biology and function of IgG4 has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Human Immunoglobulin Master Buffer Kit (Cat. No. 558683).

Preparation And Storage

This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and when reconstituted in 1.0 ml Assay Diluent has an IgG4 protein concentration of 400 ng/ml. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect PE Detection Reagent from prolonged exposure to light.

Recommended Assay Procedures

The BD CBA Human IgG4 Flex Set must be used in conjunction with a BD CBA Human Immunoglobulin Master Buffer Kit (Cat. No. 558683, 100 tests), a flow cytometer, and the FCAP Array™ Software (Cat. No. 641488). Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Immunoglobulin Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human IgG4 Flex Set will be 400 ng/ml. An example standard curve is shown in Figure 1. The Human Ig Standard provided in this Flex Set is derived from human serum and contains Human IgG1, IgG2, IgG3, IgG4, IgM, IgA and IgE proteins. Only one Human Ig Standard vial is needed when preparing standard curves for a multiplex assay using multiple Human Immunoglobulin Flex Set assays. The BD CBA Human IgG4 Flex Set cannot be used in the same assay well with the following BD CBA Immunoglobulin Flex Set reagents:

Flex Set                                                        Bead Position          Catalog Number

BD CBA Human IgG1 Flex Set                             C4                              558675

BD CBA Human Total IgG Flex Set                     C6                              558679

Due to the levels of immunoglobulin protein in typical samples and specific assay conditions, the BD CBA Human IgG4 Flex Set should not be used in the same assay well with any non-BD CBA Human Immunoglobulin Flex Set reagents (such as BD CBA Mouse Soluble Protein, Human Soluble Protein or Cell Signaling Flex Sets). For an updated assay compatibility chart for the BD CBA Human Immunoglobulin Flex Sets, please refer to the BD CBA Flex Set System homepage at http://www.bdbiosciences.com/flexset.

Performance

Limit of Detection: The theoretical limit of detection is 0.08 ng/ml and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg/ml, n=30) + 2 standard deviations.

Data Sheets

558678 Rev. 3
Components
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Description Quantity/Size Part Number EntrezGene ID
N/A 100.0 N/A N/A
558678 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.