Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- CF™ is a trademark of Biotium, Inc.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Companion Products
The 657909 monoclonal antibody specifically recognizes Tetraspanin-8 (TSPAN8) that belongs to the Transmembrane 4 (Tetraspanin) superfamily (TM4SF). This molecule is also known as Transmembrane 4 superfamily member 3 (TM4SF3). TSPAN8 is a protein that spans the membrane four times with transmembrane segments and has intracellular N- and C-termini. TSPAN8 has a small and a large extracellular loop that can mediate dimerization and association with other molecules including integrins, proteases, or cell signaling receptors. TSPAN8 is variably expressed on hematopoietic progenitor cells, spermatogonial stem cells, epithelial cells, and some tumor cells. TSPAN8 is involved in epithelial wound healing and may contribute to tumor cell metastasis.
Development References (4)
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Fu NY, Rios AC, Pal B, et al. Identification of quiescent and spatially restricted mammary stem cells that are hormone responsive.. Nat Cell Biol. 2017; 19(3):164-176. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). View Reference
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Lu J, Izvolsky KI, Qian J, Cardoso WV. Identification of FGF10 targets in the embryonic lung epithelium during bud morphogenesis. J Biol Chem. 2005; 280(6):4834-4841. (Biology). View Reference
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Mutoji K, Singh A, Nguyen T, et al. TSPAN8 Expression Distinguishes Spermatogonial Stem Cells in the Prepubertal Mouse Testis. Biol Reprod. 2016; 95(6):117. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). View Reference
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Yue S, Zhao K, Erb U, Rana S, Zoller M. Joint features and complementarities of Tspan8 and CD151 revealed in knockdown and knockout models. Biochem Soc Trans. 2017; 45(2):437-447. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.