Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- CF™ is a trademark of Biotium, Inc.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Companion Products
The 268318 monoclonal antibody specifically recognizes CD204 which is also known as Scavenger receptor class A member 1 (SR-A1 or SCARA1), Scavenger receptor type A (SRA-A), Macrophage acetylated LDL receptor I and II, or Macrophage scavenger receptor types I and II. CD204 is a trimeric type II transmembrane glycoprotein that is encoded by MSR1 (Msr1 macrophage scavenger receptor 1) which belongs to the type 1 class A scavenger receptor family. The C-terminal extracellular region of CD204 contains a scavenger receptor cysteine-rich domain followed by a collagen domain and an alpha-helical coiled coil. It is expressed on monocytes, macrophages, microglia, dendritic cells, and mast cells. CD204 is involved in the uptake of various negatively-charged macromolecules including modified proteins, polysaccharides, polyribonucleic acids, and microbial products and plays a role in innate and acquired immunity.
Development References (3)
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Cavnar MJ, Zeng S, Kim TS, et al. KIT oncogene inhibition drives intratumoral macrophage M2 polarization.. J Exp Med. 2013; 210(13):2873-86. (Clone-specific: Flow cytometry). View Reference
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Freeman M, Ashkenas J, Rees DJ, et al. An ancient, highly conserved family of cysteine-rich protein domains revealed by cloning type I and type II murine macrophage scavenger receptors.. Proc Natl Acad Sci USA. 1990; 87(22):8810-4. (Biology). View Reference
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PrabhuDas MR, Baldwin CL, Bollyky PL, et al. A Consensus Definitive Classification of Scavenger Receptors and Their Roles in Health and Disease.. J Immunol. 2017; 198(10):3775-3789. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
