Preparation And Storage
Recommended Assay Procedures
For the sandwich mouse IgG3 ELISA, R2-38 mAb is optimal for capture with biotin-conjugated anti-mouse IgG3 R40-82 mAb (Cat. No. 553401) for detection and Cat. No. 553486 as the standard. For flow cytometric detection of intracytoplasmic IgG3, we recommend FITC-conjugated mAb R40-82 (Cat. No. 553403).
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Companion Products
The R2-38 antibody reacts specifically with mouse IgG3 of all strains. It does not react with other Ig isotypes nor does it react with IgG3 of M. spretus. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with R2-38 mAb.
Development References (1)
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Huang CM, Huang HJ, Lee SC. Detection of immunoglobulin heavy chain IgG3 polymorphism in wild mice with xenogeneic monoclonal antibodies. Immunogenetics. 1984; 20(5):565-575. (Immunogen). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.