PE Rat Anti-Mouse Vβ 7 T-Cell Receptor

BD Pharmingen™ PE Rat Anti-Mouse Vβ 7 T-Cell Receptor

Name: PE Rat Anti-Mouse Vβ 7 T-Cell Receptor
Brand: BD Pharmingen™
SKU: 553216

Clone TR310

(RUO)

Two-color analysis of the expression of Vβ 7 TCR on peripheral T lymphocytes. BALB/c lymph node cells were simultaneously stained with PE-conjugated TR310, FITC-conjugated RM4-5 (anti-CD4, Cat. No. 553046/553047), and FITC-conjugated 53-6.7 (anti-CD8a, Cat. No. 553030/553031) monoclonal antibodies. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).

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Product Details

Brand: BD Pharmingen™

Reactivity: Mouse (QC Testing)

Isotype: Rat F344, also known as Fischer, CDF IgG2b, κ

Immunogen: AKR/Cum Mouse spontaneous T Lymphoma Cell Line TK1

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_394713

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Companion Products

PE Rat IgG2b, κ Isotype Control RUO

Size 0.1 mg Cat No. 553989

FITC Rat Anti-Mouse CD4 RUO

Size 0.1 mg Cat No. 553046

FITC Rat Anti-Mouse CD8a RUO

Size 0.1 mg Cat No. 553030

553216 Rev. 1

Antibody Details

TR310

The TR310 monoclonal antibody specifically recognizes the Vβ 7 T-Cell Receptor (TCR) of strains having the a (e.g., C57BR, C57L, SJL), b (e.g., A, BALB/c, CBA/Ca, C3H/He, C57BL, DBA/1), and c (e.g., RIII) haplotypes of the Tcrb gene complex. Vβ 7 is among the predominant TCRs expressed by NK-T cells of C57BL/6 mice. Vβ 7 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigens encoded by Mtv-7 (Mls-1[a], Mls[a]) endogenous proviruses (e.g., AKR, CBA/J, C58, DBA/2, NZB). Activation or elimination of Vβ 7 TCR-expressing T cells by these determinants is partially dependent upon presentation by I-E. Mtv-43 and/or exogenous MMTV-SW superantigens also cause incomplete elimination of Vβ 7 TCR-bearing T cells.

553216 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

553216 Rev.1

Citations & References

View product citations for antibody "553216" on CiteAb

Development References (4)

Bendelac A. Mouse NK1+ T cells. Curr Opin Immunol. 1995; 7(3):367-374. (Biology). View Reference
Hodes RJ, Abe R. Mouse endogenous superantigens: Ms and Mls-like determinants encoded by mouse retroviruses.. Curr Protoc Immunol. 2001; Appendix 1:Appendix 1F. (Biology). View Reference
Okada CY, Holzmann B, Guidos C, Palmer E, Weissman IL. Characterization of a rat monoclonal antibody specific for a determinant encoded by the V beta 7 gene segment. Depletion of V beta 7+ T cells in mice with Mls-1a haplotype. J Immunol. 1990; 144(9):3473-3477. (Immunogen). View Reference
Shinohara K, Ikarashi Y, Maruoka H, et al. Functional and phenotypical characteristics of hepatic NK-like T cells in NK1.1-positive and -negative mouse strains. Eur J Immunol. 1999; 29(6):1871-1878. (Biology). View Reference

View All (4)

553216 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.