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Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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Companion Products
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The HMN3-133.rMAb is a recombinant monoclonal antibody derived from HMN3-133 hybridoma cells that specifically recognizes an extracellular domain of mouse Neurogenic locus notch homolog protein 3 (Notch3 or Notch 3). Notch3 is a type 1 transmembrane glycoprotein receptor that is encoded by Notch3 (notch 3) which belongs to the Notch family containing four members, Notch1-Notch4. The Notch3 precursor is cleaved in the Golgi and presents as a cell surface heterodimeric receptor. The Notch3 receptor can bind to several membrane-bound ligands including Jagged1, Jagged2 and Delta1. Upon ligand binding, Notch3 undergoes proteolytic cleavage that results in the release of the Notch intracellular domain, NICD. NICD translocates to the nucleus where it can form a transcriptional activator complex with various transcription factors. These multimeric complexes either positively or negatively regulate the expression of multiple genes including those that orchestrate many facets of embryonic development and the subsequent functioning of organ systems such as the hematopoietic, immune, nervous, and cardiovascular systems. In the immune system, Notch3 is reportedly expressed by immature thymocytes, activated peripheral CD4+ and CD8+ T cells, and some dendritic cell subsets, but weakly or not by most types of normal lymphocytes.
Development References (9)
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Joutel A, Andreux F, Gaulis S, et al. The ectodomain of the Notch3 receptor accumulates within the cerebrovasculature of CADASIL patients.. J Clin Invest. 2000; 105(5):597-605. (Biology). View Reference
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Jönsson JI, Xiang Z, Pettersson M, Lardelli M, Nilsson G. Distinct and regulated expression of Notch receptors in hematopoietic lineages and during myeloid differentiation.. Eur J Immunol. 2001; 31(11):3240-7. (Biology). View Reference
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Moriyama Y, Sekine C, Koyanagi A, et al. Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice.. Int Immunol. 2008; 20(6):763-73. (Clone-specific: Flow cytometry). View Reference
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Murata A, Yoshino M, Hikosaka M, et al. An evolutionary-conserved function of mammalian notch family members as cell adhesion molecules.. PLoS ONE. 2014; 9(9):e108535. (Clone-specific: Flow cytometry). View Reference
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Sauma D, Ramirez A, Alvarez K, Rosemblatt M, Bono MR. Notch signalling regulates cytokine production by CD8+ and CD4+ T cells.. Scand J Immunol. 2012; 75(4):389-400. (Clone-specific: Flow cytometry). View Reference
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Sekine C, Koyanagi A, Koyama N, Hozumi K, Chiba S, Yagita H. Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes. Arthritis Res Ther. 2012; 14(2):R45-R58. (Clone-specific: Flow cytometry). View Reference
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Sekine C, Moriyama Y, Koyanagi A, et al. Differential regulation of splenic CD8- dendritic cells and marginal zone B cells by Notch ligands.. Int Immunol. 2009; 21(3):295-301. (Clone-specific: Flow cytometry). View Reference
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Sekine C, Nanki T, Yagita H. Macrophage-derived delta-like protein 1 enhances interleukin-6 and matrix metalloproteinase 3 production by fibroblast-like synoviocytes in mice with collagen-induced arthritis.. Arthritis Rheumatol. 2014; 66(10):2751-61. (Clone-specific: Flow cytometry). View Reference
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Shimizu K, Chiba S, Saito T, Kumano K, Hirai H. Physical interaction of Delta1, Jagged1, and Jagged2 with Notch1 and Notch3 receptors.. Biochem Biophys Res Commun. 2000; 276(1):385-9. (Biology). View Reference
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