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BV786 Mouse Anti-Mouse NK-1.1
Multicolor flow cytometric analysis of NK-1.1 expression on mouse splenocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142), then stained with PE Hamster Anti-Mouse CD3e antibody (Cat. No. 553063/553064/561824) and with either BD Horizon™ BV786 Mouse IgG2a, κ Isotype Control (Cat. No. 565032, Left Plot) or BD Horizon™ BV786 Mouse Anti-Mouse NK-1.1 antibody (Cat. No. 568224; Right Plot) 0.5 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of NK1.1 (or Ig Isotype control staining) versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
BD Horizon™ BV786 Mouse Anti-Mouse NK-1.1
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