Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For U.S. patents that may apply, see bd.com/patents.
Companion Products
The 1C10-1F7 monoclonal antibody specifically recognizes the variable gamma 6 region of the γ subunit of the mouse γδ T cell receptor for antigen, TCR Vγ6 (using the Heilig and Tonegawa nomenclature for mouse TCR γ and δ chains). TCR Vγ6 is encoded by the Trgv6 (T cell receptor gamma, variable 6) gene element. TCR Vγ6 is expressed by a subset of TCR γδ+ thymocytes that appear from approximately embryonic day 15 to birth and express Vγ6 paired with Vδ1 γδ TCR. These invariant TCR Vγ6Vδ1+ T cells home to various tissues including the epithelia of the lungs, peritoneal cavity, uterus, testis, tongue as well as the dermis, lamina propria of the colon, and adipose tissue. They are are programmed to secrete IL-17 upon activation. The 1C10-1F7 antibody has been found useful for immunofluorescent staining and flow cytometric analysis of TCR Vγ6+ T cells from C57BL/6 and BALB/c mice. It was also found useful for immunohistochemical analysis and in vivo blocking studies of TCR Vγ6+ γδ T cell function as well. This antibody is suitable for TCR Vγ6+ thymocyte and γδ T cell separations and analyzing TCR Vγ repertoires expressed by thymocytes, peripheral T cells, and T cell hybridomas in developmental and other experimental model systems. TCR Vγ6 is referred to as TCR Vγ4 using the Garman, Doherty, and Raulet nomenclature for mouse TCR γ and δ chains.
Development References (4)
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Hatano S, Tun X, Noguchi N, et al. Development of a new monoclonal antibody specific to mouse Vγ6 chain.. Life Sci Alliance. 2019; 2(3):e201900363. (Immunogen: Flow cytometry, Functional assay, Immunofluorescence, Immunohistochemistry, In vivo exacerbation). View Reference
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Monin L, Ushakov DS, Arnesen H, et al. γδ T cells compose a developmentally regulated intrauterine population and protect against vaginal candidiasis.. Mucosal Immunol. 2020; 13(6):969-981. (Biology). View Reference
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Roark CL, Aydintug MK, Lewis J, et al. Subset-specific, uniform activation among V gamma 6/V delta 1+ gamma delta T cells elicited by inflammation.. J Leukoc Biol. 2004; 75(1):68-75. (Biology). View Reference
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Tun X, Hatano S, Mine K, et al. Vγ6+ γδ T cells are critical for protection against infection by Escherichia coli in mice.. Eur J Immunol. 2021; 51(8):2093-2096. (Clone-specific: Flow cytometry). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.