This kit enables the fixation and permeabilization of cells which is necessary for staining intracellular cytokines with fluorochrome-conjugated anti-cytokine antibodies. The kit provides two reagents, fixation/permeabilization solution and BD Perm/Wash™ Buffer. After cell fixation and permeabilization, the BD Perm/Wash™ Buffer is used to wash the cells and to dilute the anti-cytokine antibodies for staining. This kit also provides an alternative protein transport inhibitor, BD GolgiPlug™ containing brefeldin A. Sufficient BD GolgiPlug reagent is provided for treating up to 1 liter of cell culture at a cell density of up to 2 × 10⁶ cells/ml.
Components Quantity Fixation/Permeabilization solution 125 mL BD Perm/Wash™ buffer (10x) 100 mL BD GolgiPlug protein transport inhibitor containing brefeldin A 1 mL