CD279 (MIH4) PE-Cy7

BD® CD279 (MIH4) PE-Cy7

Name: CD279 (MIH4) PE-Cy7
Brand: CD279 (MIH4) PE-Cy7
SKU: 665124

Clone MIH4 (ASR)

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Catalog No:665124

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List Price:$674.00

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Product Details

Brand: BD®

Alternative Name: PD1; hPD-1; hPD-l; PDCD1; PDC1; Programmed cell death 1; SLEB2

Reactivity: Human

Isotype: Mouse IgG1, κ

Immunogen: Human PD-1 Transfected Cell Line

Application: Flow cytometry

Workshop Number: IX 37

Regulatory Status: ASR

Data Sheets

Technical Data Sheet (TDS)

Safety Data Sheet

665124 Rev. 1

Antibody Details

MIH4

The CD279 monoclonal antibody specifically binds to a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. The CD279 antigen is also known as Programmed cell death 1 (PD-1).

665124 Rev. 1

Format Details

PE-Cy7

PE-Cy7 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 781-nm. PE can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 781 nm (e.g., a 760/60-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the Red (627–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE-Cy7

Excitation Max: 496 nm, 566 nm

Emission Max: 781 nm

665124 Rev.1

Citations & References

View Development References

Development References (9)

Bennett F, Luxenberg D, Ling V, et al. Program death-1 engagement upon TCR activation has distinct effects on costimulation and cytokine-driven proliferation: attenuation of ICOS, IL-4, and IL-21, but not CD28, IL-7, and IL-15 responses. J Immunol. 2003; 170(2):711-718. View Reference
Carter L, Fouser LA, Jussif J, et al. PD-1:PD-L inhibitory pathway affects both CD4(+) and CD8(+) T cells and is overcome by IL-2. Eur J Immunol. 2002; 32:634-643. View Reference
Dorfman DM, Brown JA, Shahsafaei A, Freeman GJ. Programmed death-1 (PD-1) is a marker of germinal center-associated T cells and angioimmunoblastic T-cell lymphoma. Am J Surg Pathol. 2006; 30:802-810. View Reference
Freeman GJ, Long AJ, Iwai Y, et al. Engagement of PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation. J Exp Med. 2000; 192:1027-1034. View Reference
Kanai T, Totsuka T, Uraushihara K, et al. Blockade of B7-H1 suppresses the development of chronic intestinal inflammation. J Immunol. 2003; 171(8):4156-4163. View Reference
Latchman Y, Wood CR, Chernova T, et al. PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat Immunol. 2001; 2(3):261-268. View Reference
Nishimura H, Minato N, Nakano T, Honjo T. Immunological studies on PD-1 deficient mice: implication of PD-1 as a negative regulator for B cell responses. Int Immunol. 1998; 10(10):1563-1572. View Reference
Pauken KE, Wherry EJ. Overcoming T cell exhaustion in infection and cancer. Trends Immunol. 2015; 36(4):265-273. View Reference
Velu V, Kannanganat S, Ibegbu C, et al. Elevated expression levels of inhibitory receptor programmed death 1 on simian immunodeficiency virus-specific CD8 T cells during chronic infection but not after vaccination. J Virol. 2007; 81(11):5819-5828. View Reference

View All (9)

665124 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For In Vitro Diagnostic Use.

23-22942-00

Analyte Specific Reagent. Analytical and performance characteristics are not established.

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