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CD138 FITC
Product Details
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BD™
Syndecan; SDC; Syndecan 1; SDC1; SYND1
Human
Mouse BALB/c IgG1, κ
Human U266 and XG-1 Myeloma Cell Lines
Flow cytometry
12.5 μg/mL
20 μL
VI BP100, B005
6382
Phosphate buffered saline with gelatin and 0.1% sodium azide.
ASR


Preparation And Storage

Store vials at 2°C to 8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

347205 Rev. 1
Antibody Details
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MI15

The CD138 antibody, clone MI15, is derived from the hybridization of SP2/0 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with human cell line 8266.

The CD138 antibody recognizes syndecan-1, a member of the syndecan family of transmembrane heparin sulfate proteoglycans whose main functions are to modulate ligand-dependent activation of primary signaling receptors at the cell surface. Syndecan-1 exhibits molecular polymorphism| its core protein is a single-chain molecule of 70 to 75 kilodaltons (kDa), and its isoforms vary in molecular weight up to approximately 400 kDa.

347205 Rev. 1
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
347205 Rev.1
Citations & References
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Development References (10)

  1. Carbone A, Gloghini A, Gattei V, et al. Reed-Sternberg cells of classical Hodgkin's disease react with the plasma cell-specific monoclonal antibody B-B4 and express human syndecan-1. Blood. 1997; 89:3787-3794. (Biology).
  2. Carey DJ. Syndecans: multifunctional cell-surface co-receptors. Biochem J. 1997; 327:43846. (Biology).
  3. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  4. Clinical and Laboratory Standards Institute. 2005. (Biology).
  5. Dhodapkar MV, Abe E, Theus A, et al. Syndecan-1 is a multifunctional regulator of myeloma pathobiology: control of tumor cell survival, growth, and bone cell differentiation. Blood. 1998; 91:2679-2688. (Biology).
  6. Gaidano G, Gloghini A, Gattei V, et al. Association of Kaposi's sacroma–associated herpes virus–positive primary effusion lymphoma with expression of the CD138/syndecan-1 antigen. Blood. 1997; 90:4894-4900. (Biology).
  7. Gattei V, Godeas C, Degan M, Rossi FM, Aldinucci D, Pinto A. Characterization of anti-CD138 monoclonal antibodies as tools for investigating the molecular polymorphism of syndecan-1 in human lymphoma cells. Br J Haematol. 1999; 104(1):152-162. (Biology). View Reference
  8. Horvathova M, Gaillard JP, Liautard J, et al. Identification of novel and specific antigens of human plasma cells by mAb. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:713-714.
  9. Sebestyén A, Berczi L, Mihalik R, Paku S, Matolcsy A, Kopper L. Syndecan-1 (CD138) expression in human non-Hodgkin lymphomas. Br J Haematol. 1999; 104:412-419. (Biology).
  10. Wijdenes J, Clément C, Klein B, Dore J-M. CD138 (syndecan-1) Workshop Panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:249-252.
View All (10) View Less
347205 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.