Description
BD Retic-COUNT™ reagent is intended for determining reticulocytes as a percentage of total erythrocytes in human peripheral blood by a FACSCalibur™, FACSort™, FACScan™, or FACStrak™ flow cytometer. The Retic-COUNT reagent is for in vitro diagnostic use.
Preparation And Storage
1. For in vitro diagnostic use.
2. The Retic-COUNT reagent is light sensitive and must be stored in the dark at 2° to 8°C. Avoid unnecessary exposure of the reagent or reagent/cell mixture to light.
3. Incubation time or temperatures other than those specified can be a source of error.
4. The reagent bottle must be kept well sealed when not in use.
5. When stored at 2° to 8°C, the reagent is stable until the expiration date shown on the label. Do not use after the expiration date.
6. WARNING: The reagent solution contains a nucleotide-reactive material and can be carcinogenic. Handle with care. Avoid contact with skin and mucous membranes.
7. The Retic-COUNT reagent contains sodium azide as a preservative; however, care should be taken to avoid microbial contamination, which can cause erroneous results."
Development References (16)
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Ferguson D, Lee S-F, Gordon, P. Evaluation of reticulocyte counts by flow cytometry in a routine laboratory. Am J Hematol. 1990; 33:13-17. (Biology).
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Gustavsson P, Holm J, Wahlen A, et al. Staging of idiopathic myelofibrosis: significance of haemoglobin values and reticulocyte counts. Acta Med Scan. 1985; 218:487-91. (Biology).
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Hillman R, Finch C. Erythropoiesis: normal and abnormal. Semin Hematol. 1972; 4:327-48. (Biology).
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Jackson AL, Warner NL. Preparation, staining, and analysis by flow cytometry of peripheral blood leukocytes. In: Rose NR, Friedman H, Fahey JL, ed. Manual of Clincial Laboratory Immunology, Third Edition. Washington DC: American Society for Microbiology; 1986:226-235.
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Koepke J, Koepke J. Review: reticulocytes. Clin Lab Haem. 1986; 8:169-80. (Biology).
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Lee LG, Chen CH, Chiu LA. Thiazole orange: a new dye for reticulocyte analysis. Cytometry. 1986; 7(6):508-517. (Biology).
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Lewis R, Sweet D, ed. (NIOSH Publication 86-103). US DHHS; 1985.
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Lichtman M, ed. Hematol for Practitioners. 1978. (Biology).
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Miale J. Laboratory Medicine Hematology 5th ed. St. Louis: CV Mosby, Co; 1977.
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National Committee for Clinical Laboratory Standards. Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Peripheral Blood Lymphocytes; Tentative Guideline. 1992; 1992. (Biology).
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National Committee for Clinical Laboratory Standards. Proposed Standard: Method for Reticulocyt eCounting (H16-P). (Biology).
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Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture: Approved Standard. NCCLS document H3-A3. 1991. (Biology).
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Savage R, Skoog D, Rabinovich A. Analytical inaccuracy and imprecision in reticulocyte counting: a preliminary report from the College of American Pathologists Reticulocyte Project. Blood Cells. 1985; 11:97-112. (Biology).
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Tanke H, Rothbarth P, Vossen J, et al. Flow cytometry of reticulocytes applied to clinical hematology. Blood. 1983; 61:1091-97. (Biology).
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Tanke H, Van Vianen P, Emiliani F, et al. Changes in erythropoiesis due to radiation or chemotherapy as studied by flow cytometric determination of peripheral blood reticulocytes. Histochem. 1986; 84:544-48. (Biology).
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Williams W, Beutler E, Erslev A, Lichtman M. Hematology 3rd ed. New York: McGraw-Hill; 1983.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For In Vitro Diagnostics Use.
Documents are subject to revision without notice. Please verify you have the correct revision of the document, and always refer back to BD's eIFU website for the latest and most up to date information.