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CD3 FITC/CD16+CD56 PE/CD45 PerCP with BD Trucount™ Tubes

BD Tritest™ CD3 FITC/CD16+CD56 PE/CD45 PerCP with BD Trucount™ Tubes

(IVD)
Product Details
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BD Tritest™
Human
Flow cytometry
IVD
Buffered saline with BSA and 0.1% sodium azide.


Description

 

BD Tritest™ CD3 fluorescein isothiocyanate (FITC)/CD16+CD56 phycoerythrin (PE)/CD45 peridinin chlorophyll protein (PerCP) is a threecolor direct immunofluorescence reagent for use with a suitably equipped flow cytometer to identify and determine the percentages and absolute counts of mature human T lymphocytes (CD3+) and natural killer (NK) (CD3CD16+CD56+) lymphocyte subsets in erythrocyte-lysed whole blood. When used with BD Trucount™ tubes, absolute counts of these populations can be enumerated from a single tube.

BD Tritest reagent and BD Trucount tubes can be used with the BD FACS™ Loader. The reagent can be used with or without an isotype control.

Preparation And Storage

1. Store the reagent at 2–8°C. Do not use after the expiration date shown on the label.

2. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the reagent vial dry.

3. Store BD Trucount tubes in their original foil pouch at 2–25°C. To avoid potential condensation, open the pouch only after it has reached room temperature and carefully reseal the pouch immediately after removing a tube. Examine the desiccant each time you open the pouch. If the desiccant has turned from blue to lavender, discard the remaining tubes. Use tubes within 1 hour after removal from the foil pouch and do not use beyond the expiration date indicated on the packaging.

340403 Rev. 1
Components
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Description Clone Isotype EntrezGene ID
CD3 FITC/CD16+CD56 PE/CD45 PerCP N/A Human 916,4684
Trucount Absolute Counting Tubes IVD N/A N/A N/A
340403 Rev. 1
Citations & References
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Development References (30)

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  21. Perussia B, Starr S, Abraham S, Fanning V, Trinchieri G. Human natural killer cells analyzed by B73.1, a monoclonal antibody blocking Fc receptor functions. I. Characterization of the lymphocyte subset reactive with B73.1. J Immunol. 1983; 130(5):2133-2141. (Biology). View Reference
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  27. Schmidt RE. Non-lineage/natural killer section report: new and previously defined clusters. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:517-542.
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340403 Rev. 1

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