Power What Matters to You in Immunology Research
Join us for an exclusive educational event designed to help you streamline panel design, extract deeper insights from every sample, and harness the full power of spectral flow cytometry. Through expert insights and real-world applications, you’ll learn how to do more with less—capturing richer, more meaningful data with greater efficiency.
Where
The Inn at Longwood Medical
Conference Room: Longwood Hall
342 Longwood Avenue
Boston, MA 02115
Parking: Parking will be validated. Parking Garage is adjacent to hotel.
Agenda
| 10:00am-10:30am | Registration |
| 10:30am-11:30am | Module 1: "Identify & Design: The Power of Choice"
Discover how to select the best fluorochromes for your experiments while understanding their impact on panel performance. This module combines insights into key attributes such as brightness, spillover, stability, and background, with a structured approach for choosing reagents that aim to deliver optimal resolution of cell populations in both small and large panels.
In this module, we’ll cover:
- Fluorochrome properties that may impact panel performance, with examples.
- How to use brightness and spillover when selecting fluorochromes.
- An approach compatible with panels for conventional and spectral flow cytometry.
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| 11:30-12:30pm | Lunch |
| 12:30-1:30pm | Guest Speaker: "Profiling Innate and Adaptive Immune Responses Across Primates and Humans"
Employing novel and high-performance fluorochromes, researchers from Precision Vaccines Program at Boston Children’s Hospital in collaboration with DFCI Flow Cytometry Core have constructed a comprehensive 28-color spectral panel to profile major innate and adaptive immune cell subsets across Human, African Green Monkeys, and Olive Baboons.
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| 1:30-2:30pm | Module 2: "Reveal & Discover: The Power of Dimensions"
Explore how spectral flow cytometry combined with spatial information at the single-cell level expands the possibilities for biological insights with sub-cellular resolution. This module integrates the principles of fluorochrome selection for well-performing panels with the added dimensions from signal-derived imaging parameters to uncover hidden biology.
In this module, we’ll cover:
- How the future has changed with real-time imaging, spectral flow cytometry.
- Revealing what was missed with added dimensions from imaging parameters.
- An example of a T-bet translocation assay using 14 colors with imaging and spectral flow cytometry and 9 multiplexed samples (fluorescent cell barcoding).
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| 2:30-2:45pm | Q&A |
