Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Synuclein cDNA was isolated from Torpedo californica and found to encode a 143 amino acid neuron-specific protein. In addition, a rat brain cDNA library yielded several clones that encode different proteins with homology to Torpedo Synuclein. A 140 amino acid protein in rat is referred to as Synuclein-1. In situ hybridization shows synuclein mRNAs in discrete areas of rat brain, notably the hippocampus. In humans, two synuclein proteins of 140 and 134 amino acids were sequenced that exhibit a high degree of homology with each other (61% identity) and with previously described Synuclein proteins. The 140 amino acid protein (α-Synuclein) is the human homologue of rat Synuclein-1 and is identical to the non-amyloid-β component precursor (NACP), a presynaptic protein involved in amyloidogenesis in Alzheimer's disease (AD). The 134 amino acid protein (β-Synuclein) is the human homologue of bovine phosphoneuroprotein. These proteins are expressed in brain, primarily in presynaptic nerve terminals. Although the exact function of the Synucleins has not been determined, they have been linked to the prominent neurodegenerative disorders AD and Parkinson's disease.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (5)
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Jo E, McLaurin J, Yip CM, St George-Hyslop P, Fraser PE. alpha-Synuclein membrane interactions and lipid specificity. J Biol Chem. 2000; 275(44):34328-34334. (Clone-specific). View Reference
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Liu Y, Fallon L, Lashuel HA, Liu Z, Lansbury PT Jr. The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility. Cell. 2002; 111(2):209-218. (Clone-specific: Immunoprecipitation, Western blot). View Reference
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Maroteaux L, Campanelli JT, Scheller RH. Synuclein: a neuron-specific protein localized to the nucleus and presynaptic nerve terminal. J Neurosci. 1988; 8(8):2804-2815. (Biology). View Reference
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Ostrerova-Golts N, Petrucelli L, Hardy J, Lee JM, Farer M, Wolozin B. The A53T alpha-synuclein mutation increases iron-dependent aggregation and toxicity. J Neurosci. 2000; 20(16):6048-6054. (Clone-specific: Immunofluorescence, Western blot). View Reference
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van der Putten H, Wiederhold KH, Probst A, et al. Neuropathology in mice expressing human alpha-synuclein. J Neurosci. 2000; 20(16):6021-6029. (Clone-specific: Immunohistochemistry). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
