Purified Mouse Anti-BrdU

BD™ Purified Mouse Anti-BrdU

Name: Purified Mouse Anti-BrdU
Brand: BD™
SKU: 347580

Clone B44

(RUO (GMP))

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Product Details

Brand: BD™

Isotype: Mouse IgG1

Immunogen: Iodouridine

Application: Flow cytometry, Intracellular staining (flow cytometry)

Concentration: 25 μg/mL

Vol. Per Test: 20 μL

RRID: AB_400326

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

Preparation And Storage

The monoclonal antibody is supplied as 50 µg purified immunoglobulin in 2.0 mL (25 µg/mL) of phosphate-buffered saline. The FITC conjugate is supplied as 50 µg in 2.0 mL (25 µg/mL). Buffered saline contains gelatin and 0.1% sodium azide. The vials should be stored at 2º to 8ºC. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

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Antibody Details

B44

The Anti-BrdU antibody, clone B44, is derived from hybridization of Sp2/0-Ag14 mouse myeloma cells with spleen cells from BALB/c mice immunized with iodouridine-conjugated ovalbumin.

Bromodeoxyuridine (BrdU) is a uridine derivative that can be incorporated specifically into DNA in place of thymidine. Anti-BrdU identifies BrdU (but not thymidine) in single-stranded DNA, free BrdU, or BrdU coupled to a protein carrier. The antibody also reacts with iodouridine. DNase-I cleaves each strand of DNA at random and permits the binding of Anti-BrdU antibody to its antigen (BrdU).

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Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

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Citations & References

Development References (7)

Beisker W, Dolbeare F, Gray JW. An improved immunocytochemical procedure for high-sensitivity detection of incorporated bromodeoxyuridine. Cytometry. 1987; 8:235. (Biology).
Dolbeare F, Beisker W, Pallavicini MG, Vanderlaan M, Gray JW. Cytochemistry for bromodeoxyuridine/DNA analysis: Stoichiometry and sensitivity. Cytometry. 1985; 6:521-530. (Biology).
Dolbeare F, Gratzner H, Pallavicini MG, Gray JW. Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine. Proc Natl Acad Sci U S A. 1983; 80(18):5573-5577. (Biology). View Reference
Gratzner HG. Monoclonal antibody to 5-bromo and 5-iododeoxyuridine: A new reagent for detection of DNA replication. Science. 1982; 218:474. (Biology).
Gray JW. Monoclonal antibodies against bromodeoxyuridine (special issue). Science. 1985; 6:501-673. (Biology).
Nagashima T, Hoshino T. Rapid detection of S-phase cells by anti-bromodeoxyuridine monoclonal antibody in 9L brain tumor cells in vitro and in situ. Acta Neuropathol (Berl). 1985; 66:12. (Biology).
Pinkel D, Thompson LH, Gray JW, Vanderlaan M. Measurement of sister chromatid exchanges at very low bromodeoxyuridine substitution levels using a monoclonal antibody in Chinese hamster ovary cells. Cancer Res. 1985; 45:5795. (Biology).

View All (7)

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.