PE Rat Anti-Human CD201

Consider BD Horizon RealYellow™ 586 (RY586) Reagents, a bright and clean fluorochrome alternative to PE off the yellow-green laser. RY586 can be used alongside PE on spectral cytometers. More Info

BD Pharmingen™ PE Rat Anti-Human CD201

Name: PE Rat Anti-Human CD201
Brand: BD Pharmingen™
SKU: 557950

Clone RCR-252

(RUO)

Flow cytometric analysis of CD201 expression on ECV304 cell line. ECV304 cells were stained with either PE Rat Anti-Human CD201 (Cat. No. 557950; solid line histogram) or Purified Rat IgG1, κ Isotype Control (Cat. No. 553925; dashed line histogram). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells.

Spectrum Viewer

Protocol Library

Scientific Resources

Product Details

Brand: BD Pharmingen™

Alternative Name: EPCR; Activated protein C receptor; APC receptor; PROCR; centrocyclin

Reactivity: Human (QC Testing)

Isotype: Rat WI, also known as Wistar (outbred) IgG1, κ

Immunogen: EPCR-positive RE-1 Cells

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 20 µl

Workshop Number: VII 70424

RRID: AB_647192

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

PE Rat IgG1, κ Isotype Control RUO

Size 0.1 mg Cat No. 553925

557950 Rev. 5

Antibody Details

RCR-252

The RCR-252 monoclonal antibody specifically binds to the endothelial cell protein C receptor (EPCR), which is also known as CD201. CD201 is a 25 kDa, type I transmembrane glycoprotein, expressed on endothelial cells in arteries, veins and capillaries. CD201 binds activated protein C, a serine protease that is involved in regulating blood coagulation. EPCR is reportedly expressed on several malignant cell lines. Malignant cell lines that express EPCR can show significant levels of protein C activation and this activation can be inhibited by anti-EPCR antibody

557950 Rev. 5

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

557950 Rev.5

Citations & References

Development References (4)

Biguzzi E, Merati G, Liaw PC, et al. A 23bp insertion in the endothelial protein C receptor (EPCR) gene impairs EPCR function. Thromb Haemost. 2001; 86(4):945-948. (Biology). View Reference
Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002.
Sturn DH, Kaneider NC, Feistritzer C, Djanani A, Fukudome K, Wiedermann CJ. Expression and function of the endothelial protein C receptor in human neutrophils. Blood. 2003; 102(4):1499-1505. (Immunogen). View Reference
Tsuneyoshi N, Fukudome K, Horiguchi S, et al. Expression and anticoagulant function of the endothelial cell protein C receptor (EPCR) in cancer cell lines. Thromb Haemost. 2001; 85(2):356-361. (Biology). View Reference

View All (4)

557950 Rev. 5

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.