Purified Mouse Anti-BrdU
Clone B44 (RUO (GMP))
- Concentration 25 μg/mL
- Vol. Per Test 20 μL
- Isotype Mouse BALB/c IgG1, κ
Flow cytometry (Routinely Tested)
- Immunogen Iodouridine
- Storage Buffer Phosphate buffered saline with gelatin and 0.1% sodium azide.
- Regulatory Status RUO (GMP)
Regulatory Status Legend
Anti-BrdU, clone B44, is derived from hybridization of mouse Sp2/0-Ag14 myeloma cells with spleen cells from BALB/c mice immunized with iodouridine-conjugated ovalbumin.
Bromodeoxyuridine (BrdU) is a uridine derivative that can be incorporated into DNA in place of thymidine. Anti-BrdU identifies BrdU (but not thymidine) in single-stranded DNA, free BrdU, or BrdU coupled to a protein carrier. The antibody also reacts with iodouridine.
Preparation and Storage
The monoclonal antibody is supplied as 50 µg purified immunoglobulin in 2.0 mL (25 µg/mL) of phosphate-buffered saline. The FITC conjugate is supplied as 50 µg in 2.0 mL (25 µg/mL). Buffered saline contains gelatin and 0.1% sodium azide. The vials should be stored at 2º to 8ºC. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.