BD Pharmingen™ PE Mouse Anti-Human CD182
Clone 6C6 (RUO)
Flow cytometric analysis of CD182 expression on human peripheral blood granulocytes. Whole blood was stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 555749; dashed line histogram) or PE Mouse Anti-Human CD182 (Cat. No. 555933; solid line histogram). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes.
Flow cytometric analysis of CD182 expression on human peripheral blood granulocytes. Whole blood was stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 555749; dashed line histogram) or PE Mouse Anti-Human CD182 (Cat. No. 555933; solid line histogram). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The 6C6 monoclonal antibody specifically binds to CD182. CD182 is also known as the interleukin-8 receptor B (IL-8RB), a G-protein-linked seven transmembrane domain protein, expressed on monocytes and granulocytes. It is also expressed on a subset of T cells. Both IL-8RA (CXCR1) and IL-8RB (CXCR2) are high affinity receptors for IL-8. IL-8 is an inflammatory cytokine which induces chemotaxis of neutrophils, basophils and T lymphocytes.
Development References (5)
-
Baggiolini M, Loetscher P, Moser B. Interleukin-8 and the chemokine family. Int J Immunopharmacol. 1995; 17(2):103-108. (Biology). View Reference
-
Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
-
Callard R, Gearing A. Callard R, Gearing A. The Cytokine Facts Book. San Diego: Academic Press; 1994.
-
Qin S, LaRosa G, Campbell JJ, et al. Expression of monocyte chemoattractant protein-1 and interleukin-8 receptors on subsets of T cells: correlation with transendothelial chemotactic potential. Eur J Immunol. 1996; 26(3):640-647. (Biology). View Reference
-
Wu L, Ruffing N, Shi X, et al. Discrete steps in binding and signaling of interleukin-8 with its receptor.. J Biol Chem. 1996; 271(49):31202-9. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.