BD Pharmingen™ PE Mouse Anti-Human CD106
Clone 51-10C9 (RUO)
Flow cytometric analysis of CD106 on TNF-α-activated HUVEC cell line. HUVEC cells were stimulated with Recombinant Human TNF Protein (Cat. No. 554618; 20 ng/ml), then stained with either PE Mouse IgG1, κ Isotype Control (Cat. No.555749; dashed line histogram) or PE Mouse Anti-Human CD106 (Cat. No. 555647/561679; solid line histogram). Fluorescent histograms were derived from gated events based on the light scattering characteristics for viable HUVEC cells. Flow cytometry was performed on a BD FACScan™.
Flow cytometric analysis of CD106 on TNF-α-activated HUVEC cell line. HUVEC cells were stimulated with Recombinant Human TNF Protein (Cat. No. 554618; 20 ng/ml), then stained with either PE Mouse IgG1, κ Isotype Control (Cat. No.555749; dashed line histogram) or PE Mouse Anti-Human CD106 (Cat. No. 555647/561679; solid line histogram). Fluorescent histograms were derived from gated events based on the light scattering characteristics for viable HUVEC cells. Flow cytometry was performed on a BD FACScan™.
Product Details
BD Pharmingen™
VCAM-1; Vascular cell adhesion protein 1; INCAM-100; L1CAM
Human (QC Testing)
Mouse IgG1, κ
Human VCAM-1 Recombinant Protein
Flow cytometry (Routinely Tested)
20 µl
V E112
AB_396003
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
PE Mouse IgG1, κ Isotype Control RUO
Size
100 Tests
Cat No.
555749
Recombinant Human TNF RUO
Size
10 µg
Cat No.
554618
PE Mouse Anti-Human CD106 RUO
Size
25 Tests
Cat No.
561679
561679 Rev. 7
Antibody Details
51-10C9
The 51-10C9 monoclonal antibody specifically binds to CD106. CD106 is a 100-110 kDa type I transmembrane sialoglycoprotrein that is also known as Vascular cell adhesion molecule-1 (VCAM-1) and INCAM-110. CD106 is expressed at high levels on the surface of cytokine-stimulated endothelium, and at minimal levels on unstimulated endothelium. VCAM-1 serves as a ligand for the leukocyte integrins α4β1 (CD49d/CD29 complex; VLA-4) and α4β7 (LPAM-1). The 51-10C9 monoclonal antibody inhibits the in vitro binding of lymphocytes and monocytes to VCAM-1 on stimulated endothelium.
561679 Rev. 7
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
561679 Rev.7
Citations & References
View product citations for antibody "561679" on CiteAb
Development References (4)
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Bevilacqua MP, Pober JS, Mendrick DL, Cotran RS, Gimbrone MA Jr. Identification of an inducible endothelial-leukocyte adhesion molecule. Proc Natl Acad Sci U S A. 1987; 84(24):9238-9242. (Biology). View Reference
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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Taichman DB, Cybulsky MI, Djaffar I, et al. Tumor cell surface alpha 4 beta 1 integrin mediates adhesion to vascular endothelium: demonstration of an interaction with the N-terminal domains of INCAM-110/VCAM-1. Cell Regul. 1991; 2(5):347-355. (Biology). View Reference
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van Vugt MJ, van den Herik-Oudijk IE, van de Winkle JG. Binding of PE-CY5 conjugates to the human high-affinity receptor for IgG (CD64). Blood. 1996; 88(6):2358-2361. (Immunogen). View Reference
561679 Rev. 7
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.