FITC Rat Anti-Rat CD45.1
Clone NDS58 (RUO)
- Brand BD Pharmingen™
- Alternative Name RT7.1 of LCA
- Concentration 0.5 mg/ml
- Isotype Rat AS IgG2b, κ
- Reactivity Rat (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen LEW rat thymocytes
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The NDS58 antibody reacts with all isoforms of CD45 (Leukocyte Common Antigen) on all hematopoietic cells, excluding the more mature erythroid cells, in strains expressing the RT7.1 alloantigen. The antibody does not react with the RT7.2 alloantigen. RT7.1 strains include ACI, AO, BN, DA, DZB, LEW, MAXX, PVG, SHR, and WAG; while BUF, LOU, and WF are RT7.2 strains. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the rat are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.