Description
The J90-462 monoclonal antibody specifically recognizes CD6. CD6 is a 105-130 kDa type I transmembrane glycoprotein that belongs to the scavenger receptor family. It is expressed on most thymocytes and peripheral T cells. CD6 is highly expressed on CD3-high thymocytes and expressed at lower levels on CD4+ CD8+ double positive and CD4+CD8- or CD4-CD8+ single positive thymocytes. CD6 is also reportedly expressed at low levels by some neurons. CD6 serves as a receptor for CD166, also known as Activated Leukocyte Cell Adhesion Molecule (ALCAM). CD6 plays a role in adhesion and migration during organogenesis. The CD6 intracellular domain is constitutively phosphorylated which suggests that CD6 may play a signaling role in T cell activation.
The antibody was conjugated to BD Horizon Red 718, which has been developed exclusively for BD Biosciences as a better alternative to Alexa Fluor® 700. BD Horizon Red 718 can be excited by the red laser (628 – 640 nm) and, with an Em Max around 718 nm, it can be detected using a 730/45 nm filter. Due to similar excitation and emission properties, we do not recommend using R718 in combination with APC-R700 or Alexa Fluor® 700.
Format
- Format
R718
- Excitation Max
718 nm
- Emission Max
695 nm
BD Horizon™ Red 718 (R718) is a small molecule fluorochrome with an emission max near 718 nm. R718 can be used as an alternative for Alexa Fluor® 700 or APC-R700 on instruments equipped with a red laser (628 – 640 nm) and a 730/45 nm filter. R718 is a bright dye with low non-specific background staining. As such, R718 offers great resolving power on surface and intracellular markers. R718 enables the resolution of low expression markers without having to switch to a tandem dye (APC-R700), which can introduce residual emission and resulting SOV/spread into the APC channel. Due to similar excitation and emission properties, we do not recommend using R718 in combination with APC-R700 or Alexa Fluor® 700. R718 has been exclusively developed for BD Life Sciences.
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
Product Notices
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Alexa Fluor® is a registered trademark of Life Technologies Corporation.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
