Purified Rat Anti-Human Cutaneous Lymphocyte Antigen
Clone HECA-452 (RUO)
- Brand BD Pharmingen™
- Alternative Name CLA; PSGL-1; CD162; P-selectin glycoprotein ligand 1; SELPL; SELPLG
- Concentration 0.5 mg/ml
- Isotype Rat WI, also known as Wistar (outbred) IgM, κ
- Reactivity Human (QC Testing) Mouse (Reported)
Flow cytometry (Routinely Tested)
Immunohistochemistry-frozen, Immunohistochemistry-formalin (antigen retrieval required) (Reported)
- Immunogen Lymphocyte-depleted Stromal Preparation of Tonsil
- Workshop No. V S075
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The HECA-452 monoclonal antibody specifically reacts with Cutaneous Lymphocyte Associated Antigen (CLA), a carbohydrate domain shared by sialyl Lewis[x] (sLe[x]) and sialyl Lewis[a] (sLe[a]) antigens. It serves as a ligand for selectins including CD62E (E-selectin; ELAM-1). CLA is expressed on high endothelium and on lymphocytes including most T lymphocytes infiltrating cutaneous sites of inflammation. Amongst peripheral blood cells, it is expressed on monocytes and granulocytes and a subset of lymphocytes. The HECA-452 antibody is also reportedly crossreactive with the mouse CLA carbohydrate epitope that is transiently expressed by PSGL-1/CD162 on activated T cells. A number of studies suggest that CLA plays an important role in supporting leucocyte adhesive interactions and migration into extravascular tissues during inflammation.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Suggested Companion Products
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Store undiluted at 4°C.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.