BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Human Platelet GPVI
Clone HY101 (also known as HY 101; HY-101)
(RUO)
Two color flow cytometric analysis of Platelet GPVI expression on human peripheral blood platelets. Platelets were isolated from fresh whole blood and fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). After washing, the fixed platelets were stained with FITC CD41a (Cat.No. 555466/557296/56185) and either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat.No.557732; Left Panel) or Alexa Fluor® 647 GPVI antibody (Cat. No. 564701; Right Panel). Two-color flow cytometric contour plots showing the correlated expression of Platelet GPVI (or Ig isotype control staining) versus CD41a were derived from events with the forward and side light-scatter characteristics of platelets. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Two color flow cytometric analysis of Platelet GPVI expression on human peripheral blood platelets. Platelets were isolated from fresh whole blood and fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). After washing, the fixed platelets were stained with FITC CD41a (Cat.No. 555466/557296/56185) and either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat.No.557732; Left Panel) or Alexa Fluor® 647 GPVI antibody (Cat. No. 564701; Right Panel). Two-color flow cytometric contour plots showing the correlated expression of Platelet GPVI (or Ig isotype control staining) versus CD41a were derived from events with the forward and side light-scatter characteristics of platelets. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Product Details
BD Pharmingen™
Glycoprotein 6; GP6; Platelet glycoprotein VI; Platelet collagen receptor
Human (QC Testing)
Mouse IgG1, κ
Glycoprotein VI Recombinant Protein
Flow cytometry (Routinely Tested)
5 µl
AB_2738900
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Fixation Buffer RUO
Size
100 mL
Cat No.
554655
FITC Mouse Anti-Human CD41a RUO
Size
100 Tests
Cat No.
555466
FITC Mouse Anti-Human CD41a RUO
Size
50 Tests
Cat No.
557296
FITC Mouse Anti-Human CD41a RUO
Size
25 Tests
Cat No.
561851
564701 Rev. 1
Antibody Details
HY101
The HY101 monoclonal antibody specifically binds to a collagen receptor, Platelet Glycoprotein VI (GPVI or GP6). GPVI is a 58-kD platelet membrane glycoprotein that belongs to the immunoglobulin superfamily. Upon blood vessel wall injury and consequent endothelial cell damage, platelets collect upon the exposed collagen fibers present in the subendothelial matrix. The platelet and collagen interaction leads to activated platelet aggregation and thrombus formation. Although normally serving a protective function in clot formation that limits blood loss, the platelet GPVI/collagen interaction may also play a role in myocardial infarction and stroke which are associated with the rupture of atherosclerotic plaques.
564701 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
564701 Rev.1
Citations & References
Development References (4)
-
Chen H, Locke D, Liu Y, Liu C, Kahn ML. The platelet receptor GPVI mediates both adhesion and signaling responses to collagen in a receptor density-dependent fashion. J Biol Chem. 2002; 277(4):3011-3019. (Immunogen: Flow cytometry, Immunoprecipitation, Radioimmunoassay, Western blot). View Reference
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Ezumi Y, Uchiyama T, Takayama H.. Molecular cloning, genomic structure, chromosomal localization, and alternative splice forms of the platelet collagen receptor glycoprotein VI. Biochem Biophys Res Commun. 2000; 277(1):27-36. (Biology). View Reference
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Hermans C, Wittevrongel C, Thys C, Smethurst PA, Van Geet C, Freson K. A compound heterozygous mutation in glycoprotein VI in a patient with a bleeding disorder. J Thromb Haemost. 2009; 7(8):1356-1363. (Clone-specific: Flow cytometry). View Reference
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Jandrot-Perrus M, Busfield S, Lagrue AH, et al. Cloning, characterization, and functional studies of human and mouse glycoprotein VI: a platelet-specific collagen receptor from the immunoglobulin superfamily. Blood. 2000; 96(5):1798-1807. (Biology). View Reference
564701 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
