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      Alexa Fluor® 647 Mouse Anti-Human CD199 (CCR9)
      Alexa Fluor® 647 Mouse Anti-Human CD199 (CCR9)
      Flow cytometric staining profile of CD199 (CCR9) expression on MOLT-4 cells. MOLT-4 cells were stained with Alexa Fluor® 647 Mouse Anti-Human CD199 (CCR9) antibody (Cat. No. 557975) or Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; shaded histogram). Fluorescence histograms were derived from gated events with the side and light scatter characteristics of viable cells.
      Alexa Fluor® 647 Mouse Anti-Human CD199 (CCR9)
      Flow cytometric staining profile of CD199 (CCR9) expression on MOLT-4 cells. MOLT-4 cells were stained with Alexa Fluor® 647 Mouse Anti-Human CD199 (CCR9) antibody (Cat. No. 557975) or Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; shaded histogram). Fluorescence histograms were derived from gated events with the side and light scatter characteristics of viable cells.
      Product Details
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      BD Pharmingen™
      CDw199; Chemokine (C-C motif) receptor 9; GPR-9-6; GPR28
      Human (QC Testing)
      Mouse IgG2a
      Human CCR9 Transfected Cell Line
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      VIII 80134
      AB_2073270
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
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      Recommended Assay Procedures

      Immunofluorescent staining and flow cytometric analysis: The Alexa Fluor® 647-conjugated anti-human CCR9 antibody can be used for immunofluorescent staining and flow cytometric analysis to identify and enumerate CCR9 expressing cells.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
      5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
      6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      557975 Rev. 4
      Antibody Details
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      112509

      The 112509 monoclonal antibody specifically binds to the human CCR9 chemokine receptor. CCR9 is a thymus specific CC chemokine receptor expressed at high levels by essentially all CD4+ and CD8+ T lymphocytes. CCR9 and its ligand CCL25/TECK are involved in lymphocyte recruitment in the small intestine. The immunogen used to generate the 112509 hybridoma was human CCR9-transfected cells.

      557975 Rev. 4
      Format Details
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      Alexa Fluor™ 647
      Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 647
      Red 627-640 nm
      653 nm
      669 nm
      557975 Rev.4
      Citations & References
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      Development References (2)

      1. Kunkel EJ, Campbell JJ, Haraldsen G, et al. Lymphocyte CC chemokine receptor 9 and epithelial thymus-expressed chemokine (TECK) expression distinguish the small intestinal immune compartment: Epithelial expression of tissue-specific chemokines as an organizing principle in regional immunity. J Exp Med. 2000; 192(5):761-768. (Biology). View Reference
      2. Zaballos A, Gutierrez J, Varona R, Ardavin C, Marquez G. Cutting edge: identification of the orphan chemokine receptor GPR-9-6 as CCR9, the receptor for the chemokine TECK. J Immunol. 1999; 162(10):5671-5675. (Biology). View Reference
      557975 Rev. 4

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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