Mouse IgG1 BV421
Clone X40 (ASR)
- Brand BD Horizon™
- Concentration 100 μg/mL
- Vol. Per Test 5 μL
- Isotype Mouse BALB/c IgG1, κ
Controls, Flow cytometry (Routinely Tested)
- Immunogen Keyhole limpet hemocyanin (KLH)
- Storage Buffer Phosphate buffered saline with gelatin and 0.1% sodium azide.
- Regulatory Status ASR
Regulatory Status Legend
The Mouse IgG1 antibody, clone X40, is derived from the hybridization of Sp2/0-Ag 14 mouse myeloma cells with spleen cells from BALB/c mice immunized with KLH.
The antibodies react specifically with keyhole limpet hemocyanin (KLH), an antigen not expressed on human cells or human cell lines.
BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.
Preparation and Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.
Protection of Laboratory Workers from Occupationally Acquired Infections; ApprovedGuideline—Third Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2005. CLSIdocument M29-A3.
Centers for Disease Control. Perspectives in disease prevention and health promotion update: universalprecautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and otherbloodborne pathogens in health-care settings. MMWR. 1988;37:377-388.