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      PE Mouse Anti-Human IREM-1 (CD300f)
      PE Mouse Anti-Human IREM-1 (CD300f)
      Two-parameter flow cytometric analysis of IREM-1 (CD300f) expression on human peripheral blood leucocytes. Whole blood cells were stained with either PE Mouse IgG1, κ Isotype Control  (Cat. No. 554680, Left Panel) or PE Mouse anti-Human IREM-1 (CD300f) antibody (Cat. No. 565450; Right Panel). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter flow cytometric contour plots showing the correlated expression of IREM-1 (CD300f) [or Ig Isotype control staining] versus side light-scatter signals (SSC) were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
      PE Mouse Anti-Human IREM-1 (CD300f)
      Two-parameter flow cytometric analysis of IREM-1 (CD300f) expression on human peripheral blood leucocytes. Whole blood cells were stained with either PE Mouse IgG1, κ Isotype Control  (Cat. No. 554680, Left Panel) or PE Mouse anti-Human IREM-1 (CD300f) antibody (Cat. No. 565450; Right Panel). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter flow cytometric contour plots showing the correlated expression of IREM-1 (CD300f) [or Ig Isotype control staining] versus side light-scatter signals (SSC) were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
      Product Details
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      BD Pharmingen™
      CD300F; CD300LF; IREM1; NKIR; CLM-1; CLM1; IGSF13
      Human (QC Testing)
      Mouse BALB/c IgG1, κ
      Human Recombinant IREM-1 Protein
      Flow cytometry (Routinely Tested)
      5 µl/test
      AB_2739243
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      6. An isotype control should be used at the same concentration as the antibody of interest.
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      565450 Rev. 2
      Antibody Details
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      UP-D1

      The UP-D1 monoclonal antibody specifically binds to Immune receptor expressed on myeloid cells 1 (IREM-1). IREM-1 is encoded by CD300LF. It is also known as CD300 antigen-like family member F (CD300f), CMRF35-like molecule 1 (CLM-1/CLM1), NK inhibitory receptor (NKIR), or Immunoglobulin superfamily member 13 (IgSF13). IREM-1/CD300f is expressed on monocytes, dendritic cells, granulocytes, and mast cells. IREM-1 is a single-pass type I transmembrane glycoprotein that belongs to the CMRF family of the Ig gene superfamily. The long intracellular region of IREM-1 contains two classical immunoreceptor tyrosine-based inhibitory motifs (ITIM). Ligand-bound IREM-1 can transduce negative regulatory signals intracellularly by recruiting SHP1 or SHIP phosphatases that in turn dephosphorylate tyrosine residues within activating cell signaling molecules. CD300f may thus play a role in regulating inflammatory responses involving myeloid cells.

              

      565450 Rev. 2
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      565450 Rev.2
      Citations & References
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      Development References (6)

      1. Aguilar H, Alvarez-Errico D, García-Montero AC, Orfao A, Sayós J, López-Botet M. Molecular characterization of a novel immune receptor restricted to the monocytic lineage. J Immunol. 2004; 173(11):6703-6711. (Biology). View Reference
      2. Alvarez-Errico D, Aguilar H, Kitzig F, Brckalo T, Sayós J, López-Botet M. IREM-1 is a novel inhibitory receptor expressed by myeloid cells. Eur J Immunol. 2004; 34(12):3690-3701. (Immunogen: ELISA, Flow cytometry, Functional assay, Immunoprecipitation, Inhibition). View Reference
      3. Alvarez-Errico D, Sayós J, López-Botet M. The IREM-1 (CD300f) inhibitory receptor associates with the p85alpha subunit of phosphoinositide 3-kinase. J Immunol. 2007; 178(2):808-816. (Clone-specific: Flow cytometry, Functional assay, Inhibition). View Reference
      4. Borrego F. The CD300 molecules: an emerging family of regulators of the immune system. Blood. 2013; 121(11):1951-1960. (Biology). View Reference
      5. Lee SM, Suk K, Lee WH. Synthetic peptides containing ITIM-like sequences of IREM-1 (CD300F) differentially regulate MyD88 and TRIF-mediated TLR signalling through activation of SHP and/or PI3K. Clin Exp Immunol. 2012; 167(3):438-446. (Biology). View Reference
      6. Sui L, Li N, Liu Q, et al. IgSF13, a novel human inhibitory receptor of the immunoglobulin superfamily, is preferentially expressed in dendritic cells and monocytes. Biochem Biophys Res Commun. 2004; 319(3):920-928. (Biology). View Reference
      View All (6) View Less
      565450 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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