Skip to main content Skip to navigation
Hamburger Menu
Search icon
Order lookup icon Order lookup icon
Order Lookup
Country Selector Country Selector
United States (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      PE Mouse Anti-PLZF
      PE Mouse Anti-PLZF
      Two-color flow cytometric analysis of PLZF expression by human peripheral blood lymphocytes and mouse thymocytes.  Left Panel: Human peripheral blood mononuclear cells were fixed and permeabilized using BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722). The cells were subsequently washed with BD Perm/Wash™ Buffer (Cat. No. 554723) and stained with Alexa Fluor® 647 Mouse Anti-Human NCAM-1 (CD56) antibody (Cat. No. 563443) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-PLZF antibody (Cat. No. 564850; Right Plot). The two-color flow cytometric contour plots showing the correlated expression of CD56 versus PLZF (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Panel: BALB/c mouse thymocytes were stained with APC Hamster Anti-Mouse CD3e antibody (Cat. No. 553066/561826). The cells were washed and similarly fixed and permeabilized. The cells were then washed with BD Perm/Wash™ Buffer and stained with either PE Mouse IgG1, κ Isotype Control (Left Plot) or PE Mouse Anti-PLZF antibody (Right Plot). The two-color flow cytometric contour plots showing the correlated expression of CD3e versus PLZF (or Ig Isotype control staining) were derived from gated events with the forward and side light- scatter characteristics of intact thymocytes. Flow cytometric analyses were performed using a BD LSRFortessa™ Cell Analyzer System.
      PE Mouse Anti-PLZF
      Two-color flow cytometric analysis of PLZF expression by human peripheral blood lymphocytes and mouse thymocytes.  Left Panel: Human peripheral blood mononuclear cells were fixed and permeabilized using BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722). The cells were subsequently washed with BD Perm/Wash™ Buffer (Cat. No. 554723) and stained with Alexa Fluor® 647 Mouse Anti-Human NCAM-1 (CD56) antibody (Cat. No. 563443) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-PLZF antibody (Cat. No. 564850; Right Plot). The two-color flow cytometric contour plots showing the correlated expression of CD56 versus PLZF (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Panel: BALB/c mouse thymocytes were stained with APC Hamster Anti-Mouse CD3e antibody (Cat. No. 553066/561826). The cells were washed and similarly fixed and permeabilized. The cells were then washed with BD Perm/Wash™ Buffer and stained with either PE Mouse IgG1, κ Isotype Control (Left Plot) or PE Mouse Anti-PLZF antibody (Right Plot). The two-color flow cytometric contour plots showing the correlated expression of CD3e versus PLZF (or Ig Isotype control staining) were derived from gated events with the forward and side light- scatter characteristics of intact thymocytes. Flow cytometric analyses were performed using a BD LSRFortessa™ Cell Analyzer System.
      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      Zbtb16; zinc finger and BTB domain containing 16; zinc finger protein 145
      Human (QC Testing), Mouse (Tested in Development)
      Mouse IgG1, κ
      Mouse PLZF Recombinant Protein
      Intracellular staining (flow cytometry) (Routinely Tested)
      0.2 mg/ml
      AB_2738984
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
      Show More blue down arrow Show Less blue up arrow

      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
      6. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      Show More blue down arrow Show Less blue up arrow
      564850 Rev. 2
      Antibody Details
      Down Arrow Up Arrow
      R17-809

      The R17-809 monoclonal antibody recognizes promyelocytic leukemia zinc finger protein (PLZF). PLZF is also known as zinc finger and BTB domain containing 16 (ZBTB16) and Zinc finger protein 145. PLZF is a member of the BTB/POZ-ZF family of transcription factors that includes Th-POK (Zbtb7b) which is involved in CD4+ T cell fate determination. PLZF serves multiple functions. In the immune system, PLZF is involved in the developmental regulation of innate immune lymphocytes including NKT cells, a subset of γδ T cells, and mucosal associated invariant T (MAIT) cells. The R17-809 hybridoma was generated from a mouse immunized with recombinant mouse PLZF protein. The R17-809 antibody crossreacts with both mouse and human PLZF.

      564850 Rev. 2
      Format Details
      Down Arrow Up Arrow
      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      564850 Rev.2
      Citations & References
      Down Arrow Up Arrow

      Development References (3)

      1. Alonzo ES, Gottschalk RA, Das J, et al. Development of promyelocytic zinc finger and ThPOK-expressing innate gamma delta T cells is controlled by strength of TCR signaling and Id3. J Immunol. 2010; 184(3):1268-1279. (Biology). View Reference
      2. Alonzo ES, Sant'Angelo DB. Development of PLZF-expressing innate T cells. Curr Opin Immunol. 2011; 23(2):220-227. (Biology). View Reference
      3. Savage AK, Constantinides MG, Han J, et al. The transcription factor PLZF directs the effector program of the NKT cell lineage. Immunity. 2008; 29(3):391-403. (Biology). View Reference
      564850 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback