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      PE Mouse anti-PLCγ1
      PE Mouse anti-PLCγ1
      Analysis of PLCγ1 in human T leukemia cell lines.  Jurkat (ATCC TIB-152, open histogram) and J.gamma1 (PLCγ1-deficient Jurkat, ATCC CRL-2678, shaded histogram) were fixed with pre-warmed BD Cytofix™ buffer (Cat. No. 554655) for 10 minutes at 37ºC, permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050) on ice for at least 30 minutes, and then stained with PE Mouse anti-PLCγ1 (Cat. No. 558575).  Flow cytometry was performed on a BD FACSArray™ bioanalyzer system.
      PE Mouse anti-PLCγ1
      Analysis of PLCγ1 in human T leukemia cell lines.  Jurkat (ATCC TIB-152, open histogram) and J.gamma1 (PLCγ1-deficient Jurkat, ATCC CRL-2678, shaded histogram) were fixed with pre-warmed BD Cytofix™ buffer (Cat. No. 554655) for 10 minutes at 37ºC, permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050) on ice for at least 30 minutes, and then stained with PE Mouse anti-PLCγ1 (Cat. No. 558575).  Flow cytometry was performed on a BD FACSArray™ bioanalyzer system.
      Product Details
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      BD Phosflow™
      Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
      Mouse IgG1
      Cow PLCγ1 N-terminal region Peptide
      Intracellular staining (flow cytometry) (Routinely Tested)
      20 µl
      AB_647225
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      558575 Rev. 2
      Antibody Details
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      10/PLCgamma

      The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol.  The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C.  Within the PLC family, PLCγ is the only member that contains SH2 and SH3 domains.  These domains enable it to interact with receptor tyrosine kinases and become enzymatically activated via phosphorylation.  It exists as two isoforms: 1) PLCγ1, which is ubiquitously expressed, and 2) PLCγ2, found primarily in the lymphoid system.  PLCγ is essential for growth factor-induced cell motility and mitogenesis.  PLCγ1 null mice exhibit retarded embryonic growth and lethality in midgestation.  Overexpression of PLCγ is evident in several forms of cancer, and it has been identified as a key mediator of PDGF-dependent cellular transformation.  Thus regulation of PLCγ activity by growth factors is involved in cell growth and transformation.

      The 10/PLCgamma monoclonal antibody recognizes PLCγ1, regardless of phosphorylation status.  It does not cross-react with PLCγ2.

      558575 Rev. 2
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      558575 Rev.2
      Citations & References
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      Development References (2)

      1. Chen P, Murphy-Ullrich JE, Wells A. A role for gelsolin in actuating epidermal growth factor receptor-mediated cell motility. J Cell Biol. 1996; 134(3):689-698. (Biology). View Reference
      2. Obermeier A, Tinhofer I, Grunicke HH, Ullrich A. Transforming potentials of epidermal growth factor and nerve growth factor receptors inversely correlate with their phospholipase C gamma affinity and signal activation. EMBO J. 1996; 15(1):73-82. (Biology). View Reference
      558575 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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