Skip to main content Skip to navigation
Hamburger Menu
Search icon
Order lookup icon Order lookup icon
Order Lookup
Country Selector Country Selector
United States (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      Purified Mouse anti-LAT (pY226)
      Purified Mouse anti-LAT (pY226)
      Western blot analysis of LAT (pY226) in human T lymphocytes.  Lysates from control (lanes 1-3) and activated (anti-CD3, Cat. No. 555329, plus anti-CD28, Cat. No. 555725, lanes 4-6) Jurkat T-cell leukemia were probed with purified mAb J96-1238.58.93 at concentrations of 1.0 (lanes 1 and 4), 0.5 (lanes 2 and 5), and 0.025 µg/ml (lanes 3 and 6).  LAT (pY226) is identified as a band of 38 kDa in the treated cells.
      Purified Mouse anti-LAT (pY226)
      LAT (pY226) staining on tonsil.  Fresh human tonsil was incubated in 5 mM Pervanadate solution for 2 hours, then fixed in formalin and processed.  Following antigen retrieval with BD Retrievagen A buffer (Cat. no. 550524), the sections were either left untreated (left panel) or treated with a phosphatase to eliminate all phosphorylation (right panel).  The tissue sections were stained with purified Mouse anti-LAT (pY226) with Hematoxylin counterstaining.  Original magnification: 20X.
      Purified Mouse anti-LAT (pY226) Purified Mouse anti-LAT (pY226)
      Western blot analysis of LAT (pY226) in human T lymphocytes.  Lysates from control (lanes 1-3) and activated (anti-CD3, Cat. No. 555329, plus anti-CD28, Cat. No. 555725, lanes 4-6) Jurkat T-cell leukemia were probed with purified mAb J96-1238.58.93 at concentrations of 1.0 (lanes 1 and 4), 0.5 (lanes 2 and 5), and 0.025 µg/ml (lanes 3 and 6).  LAT (pY226) is identified as a band of 38 kDa in the treated cells.
      LAT (pY226) staining on tonsil.  Fresh human tonsil was incubated in 5 mM Pervanadate solution for 2 hours, then fixed in formalin and processed.  Following antigen retrieval with BD Retrievagen A buffer (Cat. no. 550524), the sections were either left untreated (left panel) or treated with a phosphatase to eliminate all phosphorylation (right panel).  The tissue sections were stained with purified Mouse anti-LAT (pY226) with Hematoxylin counterstaining.  Original magnification: 20X.
      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      Human (QC Testing)
      Mouse BALB/c IgG1, κ
      Phosphorylated Human LAT Peptide
      Western blot (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required) (Tested During Development)
      38 kDa
      0.5 mg/ml
      AB_647281
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      Show More blue down arrow Show Less blue up arrow
      558363 Rev. 1
      Antibody Details
      Down Arrow Up Arrow
      J96-1238.58.93

      Engagement of the T cell receptor (TCR) induces signal transduction pathways that enhance gene transcription and cellular proliferation and differentiation.  TCR ligation results in the recruitment and activation of multiple protein tyrosine kinases (PTKs), including lck, fyn, and ZAP70.  Adaptor proteins, such as Grb2 and SLP-76, relay the signal to downstream effector molecules.  LAT (linker for activation of T cells) is a substrate of the activated ZAP70 and functions to bridge the activated TCR and its associated PTKs with tyrosine kinase substrates.  LAT is expressed as 36- and 38-kDa forms that result from post-translational modification, and as a 42-kDa form that results from alternative splicing.  LAT is an integral membrane protein that is phosphorylated at five tyrosine sites upon TCR ligation.  Following phosphorylation, LAT binds a number of important signaling molecules, including Grb2, Vav, PLCγ1, and the p85 subunit of PI3K.  Multiple studies have shown that functional LAT is required for T lymphocyte activation and thymocyte development.

      The J96-1238.58.93 monoclonal antibody recognizes the phosphorylated tyrosine 226 (pY226) of LAT, which is one of the phosphotyrosine sites required for binding Vav, Grb2, and Gads.

      558363 Rev. 1
      Format Details
      Down Arrow Up Arrow
      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      558363 Rev.1
      Citations & References
      Down Arrow Up Arrow

      Development References (5)

      1. Janssen E, Zhang W. Adaptor proteins in lymphocyte activation. Curr Opin Immunol. 2003; 15:269-276. (Biology). View Reference
      2. Lin J, Weiss A. Identification of the minimal tyrosine residues required for linker for activation of T cell function. J Biol Chem. 2001; 276:29588-29595. (Biology). View Reference
      3. Paz PE, Wang S, Clarke H, Lu X, Stokoe D, Abo A. Mapping the ZAP-70 phosphorylation sites on LAT (linker for activation of T cells) required for recruitment and activation of signalling proteins in T cells. Biochem J. 2001; 356:461-471. (Biology). View Reference
      4. Samelson LE. Signal transduction mediated by the T cell antigen receptor: The role of adapter proteins. Annu Rev Immunol. 2002; 20:371-394. (Biology).
      5. Zhu M, Janssen E, Zhang W. Minimal requirement of tyrosine residues of linker for activation of T cells in TCR signaling and thymocyte development. J Immunol. 2003; 170:325-333. (Biology). View Reference
      View All (5) View Less
      558363 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback