PE Mouse Anti-Human CD158a

BD Pharmingen™ PE Mouse Anti-Human CD158a

Name: PE Mouse Anti-Human CD158a
Brand: BD Pharmingen™
SKU: 556063

Clone HP-3E4

(RUO)

Profile of CD158 expressed on peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA)

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Product Details

Brand: BD Pharmingen™

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgM

Immunogen: Human NK Cells

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 20 µl

Workshop Number: VI BP373, NKNK14

RRID: AB_396332

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Companion Products

PE Mouse IgM, κ Isotype Control RUO

Size 100 Tests Cat No. 555584

556063 Rev. 8

Antibody Details

HP-3E4

The HP-3E4 monoclonal antibody recognizes CD158a. CD158a is a 58 kDa membrane glycoprotein, member of the killer-inhibitory receptor (KIR). The KIR family consists of transmembrane glycoproteins of the Ig superfamily expressed on <1-8% of NK cells and a subset of T cells which are involved in recognition of MHC class I molecules on target cells and inhibit cytotoxicity. CD158a is an antigen which recognizes HLA-C molecules (-Cw*0401 and -Cw*1503). This antibody is suitable for staining acetone-fixed, frozen tissue sections. It was reported that this clone also crossreacted with NKAT8 (CD158i) transfected cells.

556063 Rev. 8

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

556063 Rev.8

Citations & References

Development References (2)

Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
Melero I, Salmeron A, Balboa MA, Aramburu J, Lopez-Botet M. Tyrosine kinase-dependent activation of human NK cell functions upon stimulation through a 58-kDa surface antigen selectively expressed on discrete subsets of NK cells and T lymphocytes. J Immunol. 1994; 152(4):1662-1673. (Clone-specific: Flow cytometry, Immunohistochemistry). View Reference

556063 Rev. 8

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.