PE Hamster Anti-Mouse Vδ 6.3/2 TCR

BD Pharmingen™ PE Hamster Anti-Mouse Vδ 6.3/2 TCR

Name: PE Hamster Anti-Mouse Vδ 6.3/2 TCR
Brand: BD Pharmingen™
SKU: 555321

Clone 8F4H7B7

(RUO)

Two-color analysis of Vδ 6.3 TCR expression on intestinal intraepithelial lymphocytes (IEL). C57BL/6 IEL were simultaneously stained with FITC-conjugated mAb GL3 (antimouse γδ TCR, Cat. no. 553177, both panels) and PE-conjugated mAb 8F4H7B7 (Right panel). Flow cytometry was performed on a BD FACScan™ Flow Cytometry System (BD Biosciences, San Jose, CA).

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Product Details

Brand: BD Pharmingen™

Reactivity: Mouse (QC Testing)

Isotype: Armenian Hamster IgG2, κ

Immunogen: 1E6.11.D7, a C57BL/6 mouse Vγ1.1Vδ6.3 TCR-bearing IEL/T-cell hybridoma

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_395729

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Companion Products

PE Hamster IgG2, κ Isotype Control RUO

Size 0.1 mg Cat No. 550085

FITC Hamster Anti-Mouse γδ T-Cell Receptor RUO

Size 0.5 mg Cat No. 553177

555321 Rev. 5

Antibody Details

8F4H7B7

The 8F4H7B7 antibody reacts with Vδ 6.3/2 T-cell Receptor (TCR)-bearing T lymphocytes. Originally defined as a member of the Vδ 6 TCR subfamily, it is now proposed that the C57BL-derived Vδ 6.3 is an allelic variant of Vδ 6.2, found in A/J, AKR, BALB/c, C3H/He, and FVB mice. mAb 8F4H7B7 crossreacts with Vδ 6.4 and possibly Vδ 6.6 in DBA/2 mice, and it also detects a subset of γδ TCR-bearing cells in CBA/J and C57L mice. It does not recognize Vδ 4, Vδ 5, Vδ 6.1, or Vδ 6.5 TCR. A subpopulation of thymocytes expressing Vδ 6.3 or Vδ 6.4 TCR (in C57BL/6 or DBA/2 mice, respectively) and low levels of CD90.2 (Thy-1.2) shares functional and phenotypic characteristics with NK-T cells. Similar δV 6.4 TCRexpressing lymphocytes make up significant proportions of the γδ T-cell populations in the liver and spleen of DBA/2 mice. Furthermore, T lymphocytes bearing Vδ 6.3/2 TCR are found in the skin and intestinal epithelium and may represent a unique T-cell subpopulation with a potential for autoimmune reactivity.

555321 Rev. 5

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

555321 Rev.5

Citations & References

Development References (5)

Azuara V, Grigoriadou K, Lembezat MP, Nagler-Anderson C, Pereira P. Strain-specific TCR repertoire selection of IL-4-producing Thy-1 dull gamma delta thymocytes. Eur J Immunol. 2001; 31(1):205-214. (Immunogen). View Reference
Azuara V, Lembezat MP, Pereira P. The homogeneity of the TCRdelta repertoire expressed by the Thy-1dull gammadelta T cell population is due to cellular selection. Eur J Immunol. 1998; 28(11):3456-3467. (Biology). View Reference
Gerber DJ, Azuara V, Levraud JP, Huang SY, Lembezat MP, Pereira P. IL-4-producing gamma delta T cells that express a very restricted TCR repertoire are preferentially localized in liver and spleen. J Immunol. 1999; 163(6):3076-3082. (Biology). View Reference
Kalataradi H, Eyster CL, Fry A. Allelic differences in TCR gamma-chains alter gamma delta T cell antigen reactivity. J Immunol. 1994; 153(4):1455-1465. (Biology). View Reference
Nagler-Anderson C, McNair LA, Cradock A. Self-reactive, T cell receptor-gamma delta+, lymphocytes from the intestinal epithelium of weanling mice. J Immunol. 1992; 149(7):2315-2322. (Biology). View Reference

View All (5)

555321 Rev. 5

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.