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      Purified Mouse Anti-Rat CD8b
      Purified Mouse Anti-Rat CD8b
      The expression of CD8b on rat splenocytes. Single-cell suspensions of Lewis splenocytes were simultaneously stained with purified anti-mouse CD8a mAb G28 (Cat. No. 559977) and purified mAb 341 (Right panel), followed by biotinylated anti- mouse IgG2a mAb R19-15 (Cat. no. 553388), FITC- conjugated anti-mouse IgG1 mAb A85-1 (Cat. no. 553443), and Streptavidin-PE (Cat. no. 554061). Note that the CD8adimCD8b- population represents NK cells. Flow cytometry was performed on a BD  FACScan™ flow cytometry system.
      Purified Mouse Anti-Rat CD8b
      The expression of CD8b on rat splenocytes. Single-cell suspensions of Lewis splenocytes were simultaneously stained with purified anti-mouse CD8a mAb G28 (Cat. No. 559977) and purified mAb 341 (Right panel), followed by biotinylated anti- mouse IgG2a mAb R19-15 (Cat. no. 553388), FITC- conjugated anti-mouse IgG1 mAb A85-1 (Cat. no. 553443), and Streptavidin-PE (Cat. no. 554061). Note that the CD8adimCD8b- population represents NK cells. Flow cytometry was performed on a BD  FACScan™ flow cytometry system.
      Product Details
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      BD Pharmingen™
      Rat (QC Testing)
      Mouse BALB/c IgG1, κ
      CD8-positive Wistar rat splenic T-cell hybridomas
      Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Blocking, Immunoprecipitation, Stimulation, Western blot (Reported)
      0.5 mg/ml
      AB_395620
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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      Recommended Assay Procedures

      Other reported applications include immunoprecipitation,  western blot analysis,  in vitro blocking of allogeneic responses,  immunohistochemical

      staining of acetone-fixed frozen and zinc-fixed paraffin-embedded sections,  and stimulation of macrophages.  

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      554971 Rev. 13
      Antibody Details
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      341

      The 341 monoclonal antibody specifically recognizes the β chain of the CD8 antigen on most thymocytes and a subpopulation of mature T lymphocytes (ie, MHC class I-restricted T cells, including most T suppressor/cytotoxic cells). The CD8 α and β chains (CD8a and CD8b, respectively) form a heterodimer on the surface of most thymocytes and thymus-dependent T suppressor/cytotoxic cells, whereas intestinal intraepithelial lymphocytes, many CD8+ T cells of athymic rats, many activated CD4+ T cells, and most NK cells express CD8a without CD8b.  It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to thymus-derived T lymphocytes.  CD8 is an antigen co-receptor on the T cell surface which interacts with MHC class I molecules on antigen-presenting cells.  It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck.  Macrophages have also been reported to express CD8 α and β chains, which are involved in signal transduction.  The 341 mAb blocks proliferative and cytotoxic in vitro responses of CD8+ effectors to allogeneic cells.

      554971 Rev. 13
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      554971 Rev.13
      Citations & References
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      Development References (6)

      1. Bierer BE, Sleckman BP, Ratnofsky SE, Burakoff SJ. The biologic roles of CD2, CD4, and CD8 in T-cell activation. Annu Rev Immunol. 1989; 7:579-599. (Biology). View Reference
      2. Hirji N, Lin TJ, Befus AD. A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production. J Immunol. 1997; 158(4):1833-1840. (Clone-specific: Stimulation). View Reference
      3. Hirji N, Lin TJ, Bissonnette E, Belosevic M, Befus AD. Mechanisms of macrophage stimulation through CD8: macrophage CD8alpha and CD8beta induce nitric oxide production and associated killing of the parasite Leishmania major. J Immunol. 1998; 160(12):6004-6011. (Clone-specific: Stimulation). View Reference
      4. Janeway CA Jr. The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation. Annu Rev Immunol. 1992; 10:645-674. (Biology). View Reference
      5. Kuhnlein P, Park JH, Herrmann T, Elbe A, Hunig T. Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor. J Immunol. 1994; 153(3):979-986. (Biology). View Reference
      6. Torres-Nagel N, Kraus E, Brown MH, et al. Differential thymus dependence of rat CD8 isoform expression.. Eur J Immunol. 1992; 22(11):2841-2848. (Immunogen: Blocking, Immunoprecipitation, Western blot). View Reference
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      554971 Rev. 13

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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