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      Purified Rat Anti-Mouse CD122
      Product Details
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      BD Pharmingen™
      Il2rb; Il-2Rbeta; IL-2Rβ; IL-15Rbeta; IL-2/15 Receptor-beta; IL-2/15Rbeta
      Mouse (QC Testing)
      Rat IgG2a, κ
      Rat myeloma cells transfected with a truncated IL-2R β cDNA
      Flow cytometry (Routinely Tested), Immunoprecipitation (Reported)
      0.5 mg/ml
      AB_395400
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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      Recommended Assay Procedures

      The purified format of this antibody can be used in conjunction with biotinylated goat anti-rat Ig (with no cross-reactivity to mouse Ig) (Cat. No. 554014) and Streptavidin-phycoerythrin (Cat. No. 554061) in a three later staining procedure to amplify immunofluorescent signals for the flow cytometric analysis of cytokine receptor-expressing cells.

      This antibody has been reported to be useful for immunoprecipitation of CD122. Please note that this application is not routinely tested at BD Biosciences Pharmingen.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      554451 Rev. 1
      Antibody Details
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      5H4

      The 5H4 antibody monoclonal antibody specifically binds to mouse CD122. CD122 is a 70-85 kDa type I transmembrane glycoprotein of the hematopoietin receptor superfamily. CD122 is also known as IL-2 Receptor beta chain (IL-2Rβ) or IL-15 Receptor beta chain (IL-15Rβ) as it helps form signaling receptor complexes for Interleukin-2 (IL-2) or IL-15, respectively. CD122 can combine with either CD132 (γc) alone or CD132 plus CD25 (IL-2Rα) to form intermediate or high-affinity IL-2 Receptor complexes, respectively. The IL-2R β chain is constitutively expressed on NK cells and at lower levels on some thymocytes, T cells, B cells, monocytes, and macrophages. IL-2R β chain expression is upregulated by IL-2. The immunogen used to generate this hybridoma was rat myeloma cells transfected with a truncated IL-2R β cDNA. 5H4 does not block IL-2 binding to the IL-2 Receptor.

      554451 Rev. 1
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      554451 Rev.1
      Citations & References
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      Development References (3)

      1. Furse RK, Malek TR. Selection of internalization-deficient cells by interleukin-2-Pseudomonas exotoxin chimeric protein: the cytoplasmic domain of the interleukin-2 receptor beta chain does not contribute to internalization of interleukin-2. Eur J Immunol. 1993; 23(12):3181-3188. (Clone-specific: Flow cytometry). View Reference
      2. Malek TR, Furse RK, Fleming ML, Fadell AJ, He YW. Biochemical identity and characterization of the mouse interleukin-2 receptor beta and gamma c subunits. J Interferon Cytokine Res. 1995; 15(5):447-454. (Immunogen). View Reference
      3. Zola H. Detection of cytokine receptors by flow cytometry. In: Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W, ed. Current Protocols in Immunology. New York: Green Publishing Associates and Wiley-Interscience; 1995:6.21.1-6.21.18.
      554451 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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