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      Purified Mouse Anti-Rat IgG2b
      Product Details
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      BD Pharmingen™
      Ig gamma-2B; Immunoglobulin G2b; Cγ2b
      Rat (QC Testing)
      Mouse IgG2b, κ
      Pooled rat Ig
      ELISA Capture (Routinely Tested), Flow cytometry (Reported)
      0.5 mg/ml
      AB_395111
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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      Recommended Assay Procedures

      ELISA: For the rat IgG2b sandwich ELISA, purified G15-337 mAb is optimal for capture with biotin-conjugated anti-rat IgG2b RG7/11.1 mAb (Cat. No. 553898) for detection. Purified Rat IgG2b, clone A95-1, (Cat. No. 553986) may be used as the standard.

      Immunofluoresent staining and Flow cytometry:  G15-337 antibody is effective for detection of cell-surface or intracellular Ig by immunofluorescent staining with flow cytometric analysis. For flow cytometric detection of intracytoplasmic IgG2b, we recommend FITC-conjugated G15-337 mAb (Cat. No. 553884).

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      553882 Rev. 10
      Antibody Details
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      G15-337

      The G15-337 monoclonal antibody specifically recognizes rat IgG2b. It does not react with other Ig isotypes. A suspension of pooled rat Ig was used as the source of immunogen.

      553882 Rev. 10
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      553882 Rev.10
      Citations & References
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      Development References (2)

      1. Pérez-Berezo T, Franch A, Castellote C, Castell M, Pérez-Cano FJ. Mechanisms involved in down-regulation of intestinal IgA in rats by high cocoa intake.. J Nutr Biochem. 2012; 23(7):838-44. (Biology). View Reference
      2. Stenstad H, Svensson M, Cucak H, Kotarsky K, Agace WW. Differential homing mechanisms regulate regionalized effector CD8alphabeta+ T cell accumulation within the small intestine.. Proc Natl Acad Sci USA. 2007; 104(24):10122-7. (Clone-specific). View Reference
      553882 Rev. 10

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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