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      PE Mouse Anti-Mouse I-A[k]
      Product Details
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      BD Pharmingen™
      Aα[k]; H2-IA[k] alpha chain; H-2 I-A[k] alpha chain
      Mouse (QC Testing)
      Mouse BALB/c IgG2b, κ
      CKB mouse splenocytes
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      AB_394906
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      553537 Rev. 1
      Antibody Details
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      11-5.2

      The 11-5.2 monoclonal antibody specifically recognizes the α chain of the I-A[k] MHC class II alloantigen. It cross-reacts with cells from mice of the H-2[r] haplotype. mAb 11-5.2 recognizes the Ia.19 determinant, and it's reactivity is dependent upon the presence of Glu at position 75 of the Aα chain. Reactivity with other H-2 haplotypes (e.g., b, d, f, p, q, s) has not been observed.

      553537 Rev. 1
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      553537 Rev.1
      Citations & References
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      Development References (4)

      1. Devaux CA, Phillips ML, Delovitch TL. Idiotypic analysis of anti-I-Ak monoclonal antibodies. II. Detection of shared idiotopes on syngeneic BALB/c and allogeneic A.TH-derived anti-I-Ak mAb by BALB/c-derived anti-I-Ak anti-idiotypic mAb. J Immunol. 1984; 133(5):2595-2602. (Biology). View Reference
      2. Landais D, Marchetto S, Waltzinger C, Pierres M, Benoist C, Mathis D. Slot-machine mutagenesis of a polymorphic residue on the A kappa alpha-chain. J Immunol. 1988; 141(2):667-671. (Biology). View Reference
      3. Landias D, Beck BN, Buerstedde JM, et al.. The assignment of chain specificities for anti-Ia monoclonal antibodies using L cell transfectants. J Immunol. 1986; 137(9):3002-3005. (Biology). View Reference
      4. Oi VT, Jones PP, Goding JW, Herzenberg LA, Herzenberg LA. Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens. Curr Top Microbiol Immunol. 1978; 81:115-129. (Immunogen). View Reference
      View All (4) View Less
      553537 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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