BV421 Rat Anti-Mouse CD196 (CCR6)
Clone 140706 (RUO)
- Brand BD Horizon™
- Alternative Name CCR6, BN-1, CKR-L3, DRY6, KY411, GPR-CY4, STRL22
- Vol. Per Test 5 µl
- Isotype Rat IgG2a
- Reactivity Mouse (QC Testing)
- Application
Flow cytometry (Routinely Tested)
- Immunogen Mouse CCR6-transfected Rat Cell Line
- Entrez Gene ID 12458
- Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
Description
The 140706 monoclonal antibody specifically binds to mouse CD196 (CCR6), a CC chemokine receptor that is expressed on B lymphocytes and subsets of T and dendritic cells. The known ligand for CCR6 is CCL20/MIP-3α, and together they regulate the migration and recruitment of antigen-presenting and immunocompetent cells during inflammatory and immunological responses.
The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
Format
BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.
Suggested Companion Products
PE Rat Anti-Mouse CD45R/B220 RA3-6B2 RUO
0.1 mg
Cat No: 553089
PE Rat Anti-Mouse CD45R/B220 RA3-6B2 RUO
0.2 mg
Cat No: 553090
PE Rat Anti-Mouse CD45R/B220 RA3-6B2 RUO
25 mg
Cat No: 561878
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) 2.4G2 RUO
0.1 mg
Cat No: 553141
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) 2.4G2 RUO
0.5 mg
Cat No: 553142
Lysing Buffer RUO
100 mL
Cat No: 555899
Brilliant Stain Buffer RUO
100 Tests
Cat No: 563794
Stain Buffer (FBS) RUO
500 mL
Cat No: 554656
Stain Buffer (BSA) RUO
500 mL
Cat No: 554657
BV421 Rat IgG2a, κ Isotype Control RUO
50 µg
Cat No: 562602
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
- Brilliant Violet™ 421 is a trademark of Sirigen.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.