North America
South America
Australia & New Zealand
Europe
Middle East / Africa
Asia / Pacific
×
×
PE Mouse Anti-Human CD29 MAR4 RUO

PE Mouse Anti-Human CD29 

Clone  MAR4   (RUO)

  • Brand BD Pharmingen™
  • Alternative Name ITGB1; Integrin beta-1; Integrin β1; FNRB; GPIIA; ITB1; MDF2; MSK12; VLA-4β
  • Vol. Per Test 20 µl
  • Isotype Mouse BALB/c IgG1, κ
  • Reactivity Human (QC Testing)  Rhesus, Cynomolgus, Baboon (Tested in Development) 
  • Application Flow cytometry (Routinely Tested) 
  • Immunogen Purified Human α5β1 Fibronectin Receptor
  • Workshop No. VI A093
  • Entrez Gene ID 3688 
  • Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The MAR4 monoclonal antibody specifically binds to CD29. CD29 is a 130 kDa integrin β1 subunit that is expressed as a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. The β1 subunit has a broad tissue distribution; it is expressed on lymphocytes, monocytes and weakly on granulocytes, but not on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

Format
  • Format PE
  • Excitation Source Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
  • Excitation Max 496 nm
  • Emission Max 578 nm

R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.

Other Formats →

Suggested Companion Products

PE Mouse IgG1, κ Isotype Control RUO
50 Tests Cat No: 556650

Stain Buffer (FBS) RUO
500 mL Cat No: 554656

Stain Buffer (BSA) RUO
500 mL Cat No: 554657

Lysing Solution 10X Concentrate IVD
100 Cat No: 349202

Lysing Buffer RUO
100 mL Cat No: 555899

PE Mouse Anti-Human CD29 MAR4 RUO
100 Tests Cat No: 555443

PE Mouse Anti-Human CD29 MAR4 RUO
25 Tests Cat No: 561795

Resources & Tools
 Spectrum Viewer  Panel Designer  Spectrum Viewer  Download TDS
Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Species testing during development may have been performed with a different format of the same clone. Selected applications have been tested for cross-reactivity.
  7. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.


  1. Hynes RO. Integrins: versatility, modulation, and signaling in cell adhesion. Cell. 1992; 69(1):11-25. View reference

  2. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997; .

  3. Pellegrini R, Bazzini P, Tosi E, et al. Production and characterization of two monoclonal antibodies directed against the integrin beta 1 chain. Tumori. 1992; 78(1):1-4. View reference

  4. Reimann KA, Waite BC, Lee-Parritz DE, et al. Use of human leukocyte-specific monoclonal antibodies for clinically immunophenotyping lymphocytes of rhesus monkeys. Cytometry. 1994; 17(1):102-108. View reference

  5. Sopper S, Stahl-Hennig C, Demuth M, Johnston IC, Dorries R, ter Meulen V. Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys. Cytometry. 1997; 29(4):351-362. View reference

  6. Tanaka Y, Aso M, Takada Y. CD29 Workshop Panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997; :357-360.

557332 Rev. 6
Instruments
Reagents
Applications
Sales & Support
BD BioSciences