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Purified Mouse Anti-Vimentin RV202 RUO

Purified Mouse Anti-Vimentin 

Clone  RV202   (RUO)

  • Brand BD Pharmingen™
  • Concentration 0.5 mg/ml
  • Isotype Mouse IgG1
  • Reactivity Human (QC Testing)  Rat, Mouse, Hamster, Monkey, Dog, Chicken, Rabbit, Cow, Goat (Reported) 
  • Application Western blot (Routinely Tested) 
    Immunohistochemistry, Immunofluorescence, Electron microscopy (Reported) 
  • Immunogen Purified Cow Lens Vimentin
  • Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

Intermediate filaments (IF) are a subset of cytoskeletal proteins which function to give overall structural integrity to the plasma membrane as well to organize cells into specific tissues. IF proteins can be divided into six major types based upon the similarity in sequence. Vimentin belongs to the type III category of IF proteins which are expressed in leukocytes, blood vessel endothelial cells, some epithelial cells, and mesenchymal cells. Vimentin is also expressed together with several other IF proteins during the early stages of development. Vimentin is exchanged for the tissue-specific intermediate filament type as differentiation proceeds. Vimentin migrates in SDS/PAGE as a ~57 kDa protein.

Format
  • Format Purified

Other Formats →

Suggested Companion Products

HeLa Cell Lysate RUO
500 µg Cat No: 611449

HRP Goat Anti-Mouse Ig RUO
1 mL Cat No: 554002

Resources & Tools
 Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.


  1. Broers JL, Carney DN, Klein Rot M, et al . Intermediate filament proteins in classic and variant types of small cell lung carcinoma cell lines: a biochemical and immunochemical analysis using a panel of monoclonal and polyclonal antibodies. J Cell Sci. 1986; 83:37-60. View reference

  2. Lodish HF. Molecular cell biology, 4th ed.. New York: W.H. Freeman; 2000; :795-847.

  3. Pieper FR, Schaart G, Krimpenfort PJ, et al. Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells. J Cell Biol. 1989; 108(3):1009-1024. View reference

  4. Raats JM, Pieper FR, Vree Egberts WT, Verrijp KN, Ramaekers FC, Bloemendal H. Assembly of amino-terminally deleted desmin in vimentin-free cells. J Cell Biol. 1990; 111(5):1971-1985. View reference

  5. Viebahn C, Lane EB, Ramaekers FC. Keratin and vimentin expression in early organogenesis of the rabbit embryo. Cell Tissue Res. 1988; 253(3):553-562. View reference

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