FITC Mouse Anti-Rat CD45R
Clone HIS24 (RUO)
- Brand BD Pharmingen™
- Concentration 0.5 mg/ml
- Isotype Mouse BALB/c IgG2b, κ
- Reactivity Rat (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Low-Density Cells from AO/G Rat Peyer's Patches
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The HIS24 antibody reacts with a developmentally regulated form of CD45 found on most B lymphocytes, including developing B cells in the bone marrow and peripheral B cells, but not plasma cells. The level of expression of this CD45R antigen appears to be an indicator of both maturational stages in the B-cell lineage and of functionally distinct B-cell subsets. The HIS24 mAb, in combination with other markers such as TdT and Ig expression, is effective in the identification of B-cell progenitors. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the rat are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.