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      Purified Mouse Anti-PDPK1
      Purified Mouse Anti-PDPK1
      Western blot analysis of PDPK1 on a SW-13 cell lysate (human adrenal gland carcinoma; ATCC CCL-105). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- PDPK1 antibody.
      Purified Mouse Anti-PDPK1
      Western blot analysis of PDPK1 on a SW-13 cell lysate (human adrenal gland carcinoma; ATCC CCL-105). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- PDPK1 antibody.
      Product Details
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      BD Transduction Laboratories™
      PKB Kinase, Akt Kinase, PDK1
      Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
      Mouse IgG1
      Rat PDPK1 aa. 439-555
      Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
      58-63 kDa
      250 µg/ml
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      611070 Rev. 1
      Antibody Details
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      5/PDK1

      Protein Kinase B (PKB) is downstream of phosphoinositide 3-kinase (PI3-Kinase) that functions in a critical signaling pathway for receptor-dependent cell activation. PKB activation requires the binding of lipid metabolites phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate, phospholipid products of PI3-Kinase. These lipids bind to PKB and induce a conformational change in the enzyme, resulting in its translocation to the plasma membrane. Following cell activation, PKB is phosphorylated on Thr-308 by another kinase designated as 3-Phosphoinositide-Dependent Protein Kinase 1 (PDPK1) or also known as PKB Kinase. This kinase probably represents a new family of phospholipid-dependent activities with molecular weights that range from 31 to 220 kDa.  PDPK1 consists of 559 amino acids with an N-terminal catalytic domain and a pleckstrin-homology (PH) domain located in the C-terminal region. Like PKB, PDPK1 requires the presence of phosphatidylinositol 3,4,5-trisphosphate for maximal activity. Thus, PDPK1 acts upstream of PKB and may control signals important for proliferation, apoptosis, and metabolism.

      This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

      611070 Rev. 1
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      611070 Rev.1
      Citations & References
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      Development References (5)

      1. Fujita N, Sato S, Ishida A, Tsuruo T. Involvement of Hsp90 in signaling and stability of 3-phosphoinositide-dependent kinase-1. J Biol Chem. 2002; 277(12):10346-10353. (Biology: Immunoprecipitation, Western blot).
      2. Klippel A, Kavanaugh WM, Pot D, Williams LT. A specific product of phosphatidylinositol 3-kinase directly activates the protein kinase Akt through its pleckstrin homology domain. Mol Cell Biol. 1997; 17(1):338-344. (Biology).
      3. Nakashio A, Fujita N, Rokudai S, Sato S, Tsuruo T. Prevention of phosphatidylinositol 3'-kinase-Akt survival signaling pathway during topotecan-induced apoptosis. Cancer Res. 2000; 60(18):5303-5309. (Biology: Western blot).
      4. Scheid MP, Marignani PA, Woodgett JR. Multiple phosphoinositide 3-kinase-dependent steps in activation of protein kinase B. Mol Cell Biol. 2002; 22(17):6247-6260. (Biology: Immunofluorescence, Western blot).
      5. Stokoe D, Stephens LR, Copeland T, et al. Dual role of phosphatidylinositol-3,4,5-trisphosphate in the activation of protein kinase B. Science. 1997; 277(5325):567-570. (Biology).
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      611070 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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